Protein Info for Echvi_2049 in Echinicola vietnamensis KMM 6221, DSM 17526

Annotation: tRNA-guanine transglycosylase, queuosine-34-forming

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 376 TIGR00430: tRNA-guanine transglycosylase" amino acids 9 to 373 (365 residues), 494.5 bits, see alignment E=1.8e-152 TIGR00449: tRNA-guanine family transglycosylase" amino acids 9 to 374 (366 residues), 472.3 bits, see alignment E=9.7e-146 PF01702: TGT" amino acids 13 to 372 (360 residues), 531.6 bits, see alignment E=4.8e-164

Best Hits

Swiss-Prot: 53% identical to TGT_CLOCE: Queuine tRNA-ribosyltransferase (tgt) from Clostridium cellulolyticum (strain ATCC 35319 / DSM 5812 / JCM 6584 / H10)

KEGG orthology group: K00773, queuine tRNA-ribosyltransferase [EC: 2.4.2.29] (inferred from 80% identity to dfe:Dfer_3359)

MetaCyc: 52% identical to preQ1 tRNA-ribosyltransferase (Clostridioides difficile)
tRNA-guanine transglycosylase. [EC: 2.4.2.29]

Predicted SEED Role

"tRNA-guanine transglycosylase (EC 2.4.2.29)" in subsystem Queuosine-Archaeosine Biosynthesis (EC 2.4.2.29)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.2.29

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0FZW3 at UniProt or InterPro

Protein Sequence (376 amino acids)

>Echvi_2049 tRNA-guanine transglycosylase, queuosine-34-forming (Echinicola vietnamensis KMM 6221, DSM 17526)
MKFILENTDQKSKARTGVVKTDHGDIQTPIFMPVGTAGSVKAVHQRELTYDIKAQIILGN
TYHLYLRPGLDVLEKAGGLHKFNGWNKPILTDSGGYQVFSLAGTRKITEEGVLFKSHIDG
SKHKFTPENVMDIQRSIGADIIMAFDECTPYPCEFGYARKSMEMTHRWLKRCIDQVDSTE
GKYGYSQALFPIVQGSVYKDLRKQSAEFVASCGREGNAIGGLSVGEPAEMMYEMTELVTD
ILPEDKPRYLMGVGTPANILECIALGVDMFDCVMPTRNARNGMLFTSEGIMNMRNEKWKD
DFSPIDPNINSHVSSFYSKAYLRHLTVSKEILAAQIASVHNLSFYLWLVAEAREKIKAGE
FAVWKDEMVKKVSRRL