Protein Info for Echvi_1847 in Echinicola vietnamensis KMM 6221, DSM 17526

Updated annotation (from data): periplasmic glucoside 3-dehydrogenase (lacA subunit) (EC 1.1.99.13)
Rationale: Important for utilization of various glucosides (trehalose, salicin, cellobiose, and perhaps lactose). Related to lacA of Caulobacter crescentus which is a component of lactose 3-dehydrogenase.
Original annotation: Choline dehydrogenase and related flavoproteins

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 559 PF00890: FAD_binding_2" amino acids 7 to 40 (34 residues), 20.5 bits, see alignment (E = 5e-08) PF13450: NAD_binding_8" amino acids 10 to 40 (31 residues), 23.4 bits, see alignment (E = 1.2e-08) PF00732: GMC_oxred_N" amino acids 78 to 332 (255 residues), 59.1 bits, see alignment E=1.1e-19 PF05199: GMC_oxred_C" amino acids 423 to 542 (120 residues), 78.1 bits, see alignment E=2.1e-25

Best Hits

KEGG orthology group: None (inferred from 74% identity to psn:Pedsa_3787)

MetaCyc: 70% identical to D-glucoside-3-dehydrogenase catalytic subunit (Agrobacterium fabrum)
GLUCOSIDE-3-DEHYDROGENASE-RXN [EC: 1.1.2.11]; 1.1.2.11 [EC: 1.1.2.11]; 1.1.2.11 [EC: 1.1.2.11]; 1.1.2.11 [EC: 1.1.2.11]; 1.1.2.11 [EC: 1.1.2.11]

Predicted SEED Role

"Glucose-methanol-choline (GMC) oxidoreductase:NAD binding site" in subsystem Respiratory dehydrogenases 1

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.99.13

Use Curated BLAST to search for 1.1.2.11 or 1.1.99.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0FZD8 at UniProt or InterPro

Protein Sequence (559 amino acids)

>Echvi_1847 periplasmic glucoside 3-dehydrogenase (lacA subunit) (EC 1.1.99.13) (Echinicola vietnamensis KMM 6221, DSM 17526)
MANQEYDAIVVGSGISGGWAAKELTEKGLKVLLLERGQNVEHVKDYKNATLPPWEIPHRG
RRTTEMVENHPNLKRDYVLNELVLDWWAHEDTSPYVEEKPFTWFRGYQVGGRSLLWGRQS
YRWSDLDFEANLKEGIAVDWPIRYKDIAPWYDYVEKFAGIAGNRDGLDVLPDGEFMPPFA
MNCVEKDVKERIAKHFEGKRHLINSRVANITEPLPGRPGCQARNKCWLGCPFGGYFSTQA
STLPAAMATGNLTLRPYSIVHRVLYDKDTKKATGVEIVDAETMETIEYKSKIVFLCASAL
NSAHVLMRSATDIWPEGLGSSSGELGHNVMDHHFRLGASGTVEGYDDKYYFGRRPGGIYI
PRYRNVGDDKRDYVRGFGYQGGASRSGWGRDVAEMNIGGPMKEALTEPGPWSMGMMAFGE
ILPYHENTIKISKDVKDKWGMYALVMNAEIKDNEQKMRKDMMNDAAEMLEAAGVKNIHTY
DSGYTFGQGIHEMGTARMGRDPKTSVLNENNQVWDAKNVFVTDGAAMTSAAAVNPSLTYM
ALTARAADFAVKELKKGNL