Protein Info for Echvi_1753 in Echinicola vietnamensis KMM 6221, DSM 17526

Annotation: chaperone protein DnaK

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 646 TIGR02350: chaperone protein DnaK" amino acids 3 to 599 (597 residues), 1000.4 bits, see alignment E=1.3e-305 PF00012: HSP70" amino acids 4 to 599 (596 residues), 889.9 bits, see alignment E=6.9e-272 PF06723: MreB_Mbl" amino acids 130 to 372 (243 residues), 59.7 bits, see alignment E=2.3e-20

Best Hits

Swiss-Prot: 78% identical to DNAK_CYTH3: Chaperone protein DnaK (dnaK) from Cytophaga hutchinsonii (strain ATCC 33406 / NCIMB 9469)

KEGG orthology group: K04043, molecular chaperone DnaK (inferred from 84% identity to mtt:Ftrac_1865)

MetaCyc: 63% identical to chaperone protein DnaK (Escherichia coli K-12 substr. MG1655)
Non-chaperonin molecular chaperone ATPase. [EC: 3.6.4.10, 5.6.1.7]

Predicted SEED Role

"Chaperone protein DnaK" in subsystem Heat shock dnaK gene cluster extended or Protein chaperones

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.6.4.10 or 5.6.1.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0FYC0 at UniProt or InterPro

Protein Sequence (646 amino acids)

>Echvi_1753 chaperone protein DnaK (Echinicola vietnamensis KMM 6221, DSM 17526)
MGKIIGIDLGTTNSCVAVMEGNEPVVIQNSEGRRTTPSIVAFLDNGNGERKVGDPAKRQA
ITNPANTISSVKRFMGKKFSEVSDEKKHASYKVEQGANDTVAVKIGDRSYTPQELSAMIL
QKMKSTAEDFLGQEVTEAVITVPAYFNDAERQATKEAGQIAGLDVKRIINEPTAAALAYG
MDKKDQDMKIAVYDLGGGTFDISILELGDGVFEVKSTNGDVHLGGDDFDQVIINWLADEF
KSEEDIDLKQDPMALQRLKEAAEKAKIELSSSSSTEINLPYITATQSGPKHLVRNLSRAK
FEQLSEDLVRRSLEPCKKALSDAGLSASEIDEVILVGGSTRIPKIQEEVEKFFGKKPSKG
VNPDEVVAIGAAIQGGVLTGEVKDVLLLDVTPLSLGIETMGGVSTKLIEANTTIPSKKSE
VFSTAADNQPAVDIHVLQGERPLAKDNRSIGRFQLADIPPAPRGVPQIEVTFDIDANGIL
HVSAKDKGTGKEQKIKIEASSGLSDDEIERMKKEAEANAASDKEEKEKIEKLNQADSLIF
QTEKQLKEFGDKLSDANKTNINGALEKLKTAHQSQDLAAITPAIEELNKAWEAASTEMYN
ATQGAAGAEGAAGAGAGEGASADAGAESGDGVSDVDYEEVNEEDKK