Protein Info for Echvi_1750 in Echinicola vietnamensis KMM 6221, DSM 17526

Annotation: Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 661 PF00676: E1_dh" amino acids 22 to 314 (293 residues), 216 bits, see alignment E=1.2e-67 PF13292: DXP_synthase_N" amino acids 119 to 188 (70 residues), 25.9 bits, see alignment E=1.2e-09 PF02779: Transket_pyr" amino acids 349 to 517 (169 residues), 114.6 bits, see alignment E=8.1e-37 PF02780: Transketolase_C" amino acids 533 to 648 (116 residues), 100.8 bits, see alignment E=1e-32

Best Hits

KEGG orthology group: K11381, 2-oxoisovalerate dehydrogenase E1 component [EC: 1.2.4.4] (inferred from 66% identity to mtt:Ftrac_2815)

Predicted SEED Role

"Branched-chain alpha-keto acid dehydrogenase, E1 component, alpha subunit (EC 1.2.4.4) / Branched-chain alpha-keto acid dehydrogenase, E1 component, beta subunit (EC 1.2.4.4)" in subsystem Isoleucine degradation or Leucine Degradation and HMG-CoA Metabolism or Valine degradation (EC 1.2.4.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.4.4

Use Curated BLAST to search for 1.2.4.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0FZI3 at UniProt or InterPro

Protein Sequence (661 amino acids)

>Echvi_1750 Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit (Echinicola vietnamensis KMM 6221, DSM 17526)
MAPNMVFDRKDLSDGDLLHFYEMLLMPRKIEEKMLLLLRQGKISKWFSGWGQEAISIAAV
MAMREDEFLLPMHRNLGVFTGRGLPLGKLFAQFQGKYSGFTKGRDRSFHFGSVAHHVVGM
ISHLGPQLAVADGIALASKLGGERKATLVFTGDGATSEGDFHEALNVAAVWQLPVIFVVE
HNGYGLSTPSAEQFRFKQFIDKGPGYGMEAVKVDGNNVLELYHALSGIAEDIRHRPRPFL
VEAMTYRMRGHEESSGTKYVPKAYFEEGEKYDPVRNFEDYLQEIGVLDQSAKGVIEKRLS
EQIEAGLASAFSAEFPVAGEEELADVYCPSEGKPKEPHSSATTEKRLVDAISDGLRLSMR
QFSNLVLMGQDIGEYGGAFKVTAGFLSEFGAERVRNTPLCESAIIGAALGLSVKGFKSVV
EMQFADFVSCGFNQIVNNLAKVHYRWGQHADVVIRMPTGAGVGAGPFHSQSNEAWFFHTP
GLKILYPSSPQDAKGLLAAAIEDPNPCLFFEHKALYRSVIGQVPDEYYTVEIGKAHLVKE
GDQATVVTYGMGVHWAKRVMESLDVRVDLLDLRTLLPWDKEAVEKSVKKTNKVMILHEDC
LTGGIGAEIAAWISEHCFECLDAPVMREGSLDTPVPFAANLEENFLPENRFKDKLMALLA
Y