Protein Info for Echvi_1681 in Echinicola vietnamensis KMM 6221, DSM 17526

Annotation: Glycogen debranching enzyme

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 731 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details PF21209: Bac_rhamnosid-like_N" amino acids 74 to 277 (204 residues), 310.1 bits, see alignment E=6.2e-97 PF17389: Bac_rhamnosid6H" amino acids 301 to 633 (333 residues), 91.7 bits, see alignment E=5.9e-30

Best Hits

KEGG orthology group: None (inferred from 68% identity to fjo:Fjoh_4105)

Predicted SEED Role

"Alfa-L-rhamnosidase (EC 3.2.1.40)" in subsystem L-rhamnose utilization (EC 3.2.1.40)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.40

Use Curated BLAST to search for 3.2.1.40

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0FYW4 at UniProt or InterPro

Protein Sequence (731 amino acids)

>Echvi_1681 Glycogen debranching enzyme (Echinicola vietnamensis KMM 6221, DSM 17526)
METQKYIPLLLLFILLVAPKETWAQENENEPSATWIWYPGDYEIWLSNKMQARRTERGAF
LPPLWRYYSPYALVTFRKEFTLPAEDEIAIYTEGQFKLQIDGKQQHGSSRKITIPPGDHS
IVVKVYNQERVPAVFIEGDYLVTDDSWKVTFEDKEWIDESGKASDQSGTNWEAVGTWDFN
TPEDLPSEFSLATTPQFAAAVQPAGEGQLIDFGKETFGYIQFHGLKGEGNVNIYYGESEE
EALDSAYCETLDYLSFDGSQAEDYTVQNSKAFRYVQVQHDPGVSYDSVSMLYEYLPVEYR
GEWNSSDELLNEIWDVSAYTMHLNTREFFLDGIKRDRWIWSGDAYQSYLMNYYLFFDNAS
VRRTLLALRGKEPVSSHMNTIMDYSFYWFVGIYDYYLYSGDEEFIKNFYPRMVSMMDFCL
GRRNEHGMMEGLPGDWIFIDWADGLSKQGEVSFEQMLLARSLEAMAVSAEIAGDHEGHAT
YQKLADEMKEKLFEVFWSEDREAIMHQRVDGKVLDNVTRYANMFGIFFDYFSDDQKEAVK
HSVLLNDEVQKITTPYMRFYELEALCAMGEQAFVLDEIRDYWGGMLDLGATSFWEKYDPN
ESGTEHLSMYGRPYGKSLCHAWGASPVYLFGKYYLGVKPLSAGYETYEIRPELGGLDWME
GKVPTPNGDVSVYCSTKEIKVSSDEGEGVLIFKSKSKPRTDQGEIKALGGDEYELALGAG
KEYVVKYKALK