Protein Info for Echvi_1587 in Echinicola vietnamensis KMM 6221, DSM 17526

Updated annotation (from data): Saccharopine dehydrogenase (NADP(+), L-lysine-forming) (EC 1.5.1.8); Saccharopine dehydrogenase (NAD(+), L-glutamate-forming) (EC 1.5.1.9)
Rationale: Specifically important for utilizing L-Lysine. Automated validation from mutant phenotype: the predicted function (1.5.1.8, 1.5.1.9) was linked to the condition via a SEED subsystem. This annotation was also checked manually.
Original annotation: Alanine dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

KEGG orthology group: None (inferred from 52% identity to mtt:Ftrac_2781)

Predicted SEED Role

"Lysine ketoglutarate reductase (EC 1.5.1.8) (LOR) (LKR) / Saccharopine dehydrogenase (EC 1.5.1.9)" in subsystem Lysine degradation (EC 1.5.1.8, EC 1.5.1.9)

MetaCyc Pathways

L-lysine degradation XI (3/5 steps found)
superpathway of L-lysine degradation (9/43 steps found)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.5.1.8 or 1.5.1.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0FX15 at UniProt or InterPro

Protein Sequence (404 amino acids)

>Echvi_1587 Saccharopine dehydrogenase (NADP(+), L-lysine-forming) (EC 1.5.1.8); Saccharopine dehydrogenase (NAD(+), L-glutamate-forming) (EC 1.5.1.9) (Echinicola vietnamensis KMM 6221, DSM 17526)
MKIGLIKEGKVPSDRRVAFSPQQLKTMNEAYAGRAVFVVEKSDIRAFKNEEYEEEGIEVV
DDVSDCDVLMGIKEVPIASLMEGKTYFFFSHTIKAQPYNRGLLQAVLEKDIRLVDYEVLR
NAGERVVAFGRWAGIVGGYNGLWTYGKKTGLFDMKRAKDCFDLMELHEEVKQIQLPPIKM
VITGNGRVGNGVKEILQVAGIREVSPKELLQNYYDEPVFVQLAMEDYNRRKTDGGYDKAE
FYSQPEKYESHFLKFAEVSDVLFAAAFWDPKAPKLFERKDVVSEDFNLSVIADITCDIDG
SVPTTVKPSTIDAPVYDVDRESFEVLPAFGEQLSISVMAIDNLPCELPRDASEDFGSQLM
ETVIPALLEDESGIIEKATIAENGELTHYFDYLEDFVRERSDDS