Protein Info for Echvi_1572 in Echinicola vietnamensis KMM 6221, DSM 17526
Updated annotation (from data): Lactaldehyde dehydrogenase (EC 1.2.1.22); Rhamnulose-1-phosphate aldolase (EC 4.1.2.19)
Rationale: Specifically important for utilizing L-Rhamnose monohydrate. Automated validation from mutant phenotype: the predicted function (1.2.1.22, 4.1.2.19) was linked to the condition via a SEED subsystem. This annotation was also checked manually.
Original annotation: rhamnulose-1-phosphate aldolase/alcohol dehydrogenase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: None (inferred from 72% identity to zpr:ZPR_1549)Predicted SEED Role
"Predicted rhamnulose-1-phosphate aldolase (EC 4.1.2.19) / Predicted lactaldehyde dehydrogenase (EC 1.2.1.22)" in subsystem L-rhamnose utilization (EC 1.2.1.22, EC 4.1.2.19)
MetaCyc Pathways
- L-rhamnose degradation I (5/5 steps found)
- methylglyoxal degradation V (3/3 steps found)
- methylglyoxal degradation IV (2/3 steps found)
- L-lactaldehyde degradation (aerobic) (1/2 steps found)
- superpathway of methylglyoxal degradation (5/8 steps found)
- lactate biosynthesis (archaea) (2/5 steps found)
- superpathway of fucose and rhamnose degradation (7/12 steps found)
- L-rhamnose degradation II (2/8 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.2.1.22
Use Curated BLAST to search for 1.2.1.22 or 4.1.2.19
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See L0FWZ9 at UniProt or InterPro
Protein Sequence (702 amino acids)
>Echvi_1572 Lactaldehyde dehydrogenase (EC 1.2.1.22); Rhamnulose-1-phosphate aldolase (EC 4.1.2.19) (Echinicola vietnamensis KMM 6221, DSM 17526) MSTAERTFKHVNYLWDEQKAQELEGDEVALLIYRSNILGADLRITNYGGGNTSCKTTEVD PLTKEETEVMWVKGSGGDIGTLKRSGLAGLYVEKLHSLKNVYRGLEFEDEMVGLFNHCIY DLDSKAPSIDTPLHAFLPFKHIDHLHPDAAIAIAASKDGEKITEELFEGQIAWVPWQRPG FDLALQLEKALNENPGIRGIMLGGHGLFTWGDTAYECYINSLEVIDKASEYLEQNYGKDR PVFGGQKIESLAPEQRQEQASIIAPVLRGLASGYNRMVGHFTDDERVLQFANSHDLEKLA PLGTSCPDHFLRTKIRPLVLDFPADVDLSKADEIKEKLDKDFEEYRAYYKKYYEDHKRDN SPAMRDPNPVVIIWPGVGMFSYAKNKQTARVASEFYINAINVMRGAEAVSEYVALPLQEA FDIEYWLLEEAKLQRMPKEQPLSRKVALVTGGAGGIGKAIADKLASEGACVFITDINQER LDGAVATYSKDVGGGAVMDVTKGDDIIKAYKAAALKFGGVDIIVNCAGLAISKPIEQTSE QDWDLLQDILVKGQFAVSKAGVETLRAQNLGGDIINIASKNALVSGPNNVGYGTAKAAQV HMSRLLAAELGKDKIRVNVVNPDAVIEGSKIWEGEWAKGRAKAYGITVEELPAFYAKRTI LNEIIGVDDIANGVFAFVGGHLSKCTGNILNVDGGVAAAFVR