Protein Info for Echvi_1427 in Echinicola vietnamensis KMM 6221, DSM 17526

Updated annotation (from data): putative N-succinyl-L,L-diaminopimelate desuccinylase (EC 3.5.1.18)
Rationale: This putative amidohydrolase, and several homologs, are essential, and probably replace the missing DapE
Original annotation: amidohydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01891: amidohydrolase
  • amino acids 18 to 381 (364 residues), 351.1 bits, E=3.9e-109
• PF01546: Peptidase_M20
  • amino acids 76 to 386 (311 residues), 141.1 bits, E=4.6e-45
• PF07687: M20_dimer
  • amino acids 193 to 284 (92 residues), 37 bits, E=2.8e-13

Best Hits

Swiss-Prot: 39% identical to YHAA_BACSU: Putative amidohydrolase YhaA (yhaA) from Bacillus subtilis (strain 168)

KEGG orthology group: K01436, amidohydrolase [EC: 3.5.1.-] (inferred from 69% identity to chu:CHU_0130)

MetaCyc: 39% identical to N-acetyl amino acid acetylase (Bacillus subtilis subtilis 168)
3.5.1.-

Predicted SEED Role

"N-acetyl-L,L-diaminopimelate deacetylase (EC 3.5.1.47)" in subsystem Lysine Biosynthesis DAP Pathway (EC 3.5.1.47)

MetaCyc Pathways

• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (15/18 steps found)
• aspartate superpathway (20/25 steps found)
• L-lysine biosynthesis I (8/9 steps found)
• L-lysine biosynthesis II (7/9 steps found)
• S-(2-succinyl)-L-cysteine degradation (2/4 steps found)
• S-benzyl-L-cysteine degradation (1/5 steps found)

KEGG Metabolic Maps

• 1,4-Dichlorobenzene degradation
• Caprolactam degradation
• Glutathione metabolism
• Lipopolysaccharide biosynthesis
• Lysine biosynthesis
• Nicotinate and nicotinamide metabolism
• Nucleotide sugars metabolism
• Sphingolipid metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.-, 3.5.1.47

Use Curated BLAST to search for 3.5.1.- or 3.5.1.18 or 3.5.1.47

Sequence Analysis Tools

See L0FXC2 at UniProt or InterPro

Protein Sequence (397 amino acids)

>Echvi_1427 putative N-succinyl-L,L-diaminopimelate desuccinylase (EC 3.5.1.18) (Echinicola vietnamensis KMM 6221, DSM 17526)
MLKDTIKKLAADQLNQTIAIRRHIHAHPELSFEEHQTCAFVEKHLQEIGITNIQRKANTG
LVALIEGKNPSKKVIALRADMDALPIVEQNDVPYKSNKEGVMHACGHDVHTSSLLGAASI
LHAVKDQFEGTVKLIFQPGEEKIPGGASLMIKDKALENPRPSGIVGQHVMPLIDAGKVGF
RKGMYMASADELYLKVIGKGGHGAMPETLVDPVLIASHIIVALQQVISRNASPKVPSVLS
FGRIEALGATNVIPNEVNIQGTFRTLDETWRAEAHQKMVKIAEGIAEGMGGSVDFEVRKG
YPFLQNAPELTDRAYKAAQAYLGEENVEDLDIWMAAEDFSYYTQEMDGCFYRLGIRNEEK
GITSGVHTPTFDIDESALEVGAGLMAWIAINELMSEA