Protein Info for Echvi_1262 in Echinicola vietnamensis KMM 6221, DSM 17526

Annotation: asparagine synthase (glutamine-hydrolyzing)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01536: asparagine synthase (glutamine-hydrolyzing)
  • amino acids 2 to 528 (527 residues), 355.7 bits, E=2.7e-110
• PF13522: GATase_6
  • amino acids 37 to 160 (124 residues), 106.7 bits, E=1.4e-34
• PF13537: GATase_7
  • amino acids 47 to 164 (118 residues), 106.4 bits, E=1.5e-34
• PF00733: Asn_synthase
  • amino acids 236 to 592 (357 residues), 213.3 bits, E=1.2e-66

Predicted SEED Role

"Asparagine synthetase [glutamine-hydrolyzing] (EC 6.3.5.4)" in subsystem Cyanophycin Metabolism or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 6.3.5.4)

MetaCyc Pathways

• L-citrulline biosynthesis (8/8 steps found)
• L-glutamate and L-glutamine biosynthesis (7/7 steps found)
• superpathway of L-citrulline metabolism (10/12 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (4/4 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• L-asparagine biosynthesis I (1/1 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (3/4 steps found)
• superpathway of L-asparagine biosynthesis (1/2 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Nitrogen metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.5.4

Sequence Analysis Tools

See L0FWZ1 at UniProt or InterPro

Protein Sequence (598 amino acids)

>Echvi_1262 asparagine synthase (glutamine-hydrolyzing) (Echinicola vietnamensis KMM 6221, DSM 17526)
MCGINLAMNLPGSGEDAIQQMMAATAHRGPDHSDWCKITGQLFLAGNRLKTVDLSDWSNQ
PLQINQGAHTLVWNGALYNADELRNELLEDGESFESRSDSEVLLRWLKRHGISGINRLQG
MYALVFVEREEKSIIIARDPHGKKPLYYFHQNHLWLFSSEARGIIAAGQIKRQLDKTQLT
PYLYTRHSFPDKSFFQQVQQIVPGKALQLDFGGNIRAEHRTVIPQATLELPRKEHFRSLL
TDATLKHFRADIPVGVLLSGGADSSLLLDTWMRETDLPLHTFTARFEQKYLKKYQDPIHA
RQVAEKYRCVHHEVLITPALLRAQLPEYIASLDQPVGDSASFLSWMIAREAKEHVKILIS
GAGADELFGGYNRHEAFKQYLQHKAIALKASKVMGKLSFLGRHFQKIAKGIRKDEATTFL
NFSALRNIPADQREAFLAYYPKGVPPYKAALEWDRSYYLVNDILKIHDNALMAHGVEGRA
PYLDKNLVSLSMSLTEEQHLSLKPKQWIRELLRETGLEKVAVRKKFGFGLPLKEWLEEDQ
ALRSLVVETISGFVQQHHHSIPEEFHILAKEPDKKIKLHFLEIWNLYILAAWCTYHQL