Protein Info for Echvi_1221 in Echinicola vietnamensis KMM 6221, DSM 17526

Annotation: pyruvate dehydrogenase E1 component, alpha subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR03182: pyruvate dehydrogenase (acetyl-transferring) E1 component, alpha subunit
  • amino acids 19 to 330 (312 residues), 472.6 bits, E=2.3e-146
• PF00676: E1_dh
  • amino acids 26 to 320 (295 residues), 334.3 bits, E=1.1e-103
• PF02775: TPP_enzyme_C
  • amino acids 114 to 247 (134 residues), 37.1 bits, E=5.4e-13
• PF13292: DXP_synthase_N
  • amino acids 128 to 235 (108 residues), 23.6 bits, E=5.8e-09
• PF00456: Transketolase_N
  • amino acids 149 to 250 (102 residues), 22.8 bits, E=8.7e-09

Best Hits

Swiss-Prot: 56% identical to ODPA_RICFE: Pyruvate dehydrogenase E1 component subunit alpha (pdhA) from Rickettsia felis (strain ATCC VR-1525 / URRWXCal2)

KEGG orthology group: K00161, pyruvate dehydrogenase E1 component subunit alpha [EC: 1.2.4.1] (inferred from 72% identity to lby:Lbys_2059)

MetaCyc: 47% identical to pyruvate dehydrogenase alpha1 subunit (Ascaris suum)
PYRUVDEH-RXN [EC: 1.2.1.104]

Predicted SEED Role

"Pyruvate dehydrogenase E1 component alpha subunit (EC 1.2.4.1)" in subsystem Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate (EC 1.2.4.1)

MetaCyc Pathways

• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (18/22 steps found)
• pyruvate decarboxylation to acetyl CoA I (3/3 steps found)
• TCA cycle I (prokaryotic) (8/10 steps found)
• TCA cycle III (animals) (8/10 steps found)
• TCA cycle VIII (Chlamydia) (5/6 steps found)
• 1-butanol autotrophic biosynthesis (engineered) (20/27 steps found)
• TCA cycle II (plants and fungi) (7/9 steps found)
• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (19/26 steps found)
• pyruvate fermentation to acetate V (2/3 steps found)
• superpathway of glyoxylate bypass and TCA (8/12 steps found)
• pyruvate fermentation to acetate II (1/3 steps found)
• anaerobic energy metabolism (invertebrates, mitochondrial) (6/10 steps found)
• photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (17/26 steps found)
• pyruvate fermentation to acetate and (S)-lactate I (1/4 steps found)
• superpathway of anaerobic energy metabolism (invertebrates) (10/17 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Butanoate metabolism
• Citrate cycle (TCA cycle)
• Glycolysis / Gluconeogenesis
• Pyruvate metabolism
• Valine, leucine and isoleucine biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 1.2.4.1

Use Curated BLAST to search for 1.2.1.104 or 1.2.4.1

Sequence Analysis Tools

See L0FW29 at UniProt or InterPro

Protein Sequence (340 amino acids)

>Echvi_1221 pyruvate dehydrogenase E1 component, alpha subunit (Echinicola vietnamensis KMM 6221, DSM 17526)
MAKKSSTTTKSKVKYSKETYTFWYESMLLMRRFEEKAGQLYGQQKIRGFCHLYIGQEACA
AGAITALEKDDKWITAYRDHAHPLGLGTDPGAVMAELFGKATGTTKGKGGSMHIFDKERN
FMGGHGIVGAQVPMGLGIGFAEKYKGTKNLCICHMGDGAVRQGAVHESFNLAMLYKVPVI
FVIENNGYAMGTSVKRSSNVDDLSTLGESYDMPSFAVDGMNVEEVHEAVAEAAERARKGD
GPTLLEVRTYRYKGHSMSDPQKYRTREEVEEYKAKDPIEQVKKTILDNKILSEDDIKEID
AKVKKQVADAVKFAEESPWPDGQKAFEDVYMQEDYPFVME