Protein Info for Echvi_1188 in Echinicola vietnamensis KMM 6221, DSM 17526

Annotation: Glycine/serine hydroxymethyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00464: SHMT
  • amino acids 3 to 392 (390 residues), 554.7 bits, E=9.8e-171
• PF00155: Aminotran_1_2
  • amino acids 81 to 380 (300 residues), 30 bits, E=3.2e-11

Best Hits

Swiss-Prot: 73% identical to GLYA_CYTH3: Serine hydroxymethyltransferase (glyA) from Cytophaga hutchinsonii (strain ATCC 33406 / NCIMB 9469)

KEGG orthology group: K00600, glycine hydroxymethyltransferase [EC: 2.1.2.1] (inferred from 76% identity to mtt:Ftrac_3134)

Predicted SEED Role

"Serine hydroxymethyltransferase (EC 2.1.2.1)" in subsystem Folate Biosynthesis or Glycine Biosynthesis or Glycine and Serine Utilization or LMPTP YwlE cluster or Photorespiration (oxidative C2 cycle) or Serine-glyoxylate cycle or Serine Biosynthesis (EC 2.1.2.1)

MetaCyc Pathways

• folate transformations III (E. coli) (8/9 steps found)
• superpathway of L-serine and glycine biosynthesis I (4/4 steps found)
• dTMP de novo biosynthesis (mitochondrial) (3/3 steps found)
• glycine degradation (3/3 steps found)
• folate polyglutamylation (4/5 steps found)
• glycine biosynthesis I (1/1 steps found)
• folate transformations II (plants) (8/11 steps found)
• glycine betaine degradation III (4/7 steps found)
• folate transformations I (8/13 steps found)
• glycine betaine degradation I (4/8 steps found)
• glycine betaine degradation II (mammalian) (1/4 steps found)
• photorespiration I (4/9 steps found)
• photorespiration III (4/9 steps found)
• photorespiration II (4/10 steps found)
• formaldehyde assimilation I (serine pathway) (6/13 steps found)
• purine nucleobases degradation II (anaerobic) (9/24 steps found)

KEGG Metabolic Maps

• Cyanoamino acid metabolism
• Glycine, serine and threonine metabolism
• Methane metabolism
• One carbon pool by folate

Isozymes

Compare fitness of predicted isozymes for: 2.1.2.1

Use Curated BLAST to search for 2.1.2.1

Sequence Analysis Tools

See L0FU83 at UniProt or InterPro

Protein Sequence (422 amino acids)

>Echvi_1188 Glycine/serine hydroxymethyltransferase (Echinicola vietnamensis KMM 6221, DSM 17526)
MKRDQVIFDLIQKEEDRQKRGIELIASENFTSKQVMEAAGSVLTNKYAEGLPKKRYYGGC
EVVDDIEQIAIDRAKELFGATWANVQPHSGAQANAAVFLACLNPGDHILGFDLSHGGHLT
HGSPVNFSGKNYKPHFYGVEEETGIIDMDKVAEKAREVKPKLIICGASAYSRDWDYARFR
EIADEVGALLLADISHPAGLIARGLLKDPLEHCHIVTTTTHKTLRGTRGGLIMMREDFDN
PFGIKNPKGELRKMTQLLDSAVFPGMQGGPLEHIIAAKAIAFQEALSDEYMEYILQVKKN
ASIMADAFVEKGYKLISGGTDNHLMLIDLRNKDLSGKIAEETLGKVDITINKNMVPFDTR
SPFVTSGMRVGTAAITSRGLKEQEMTKIVDLIDRALMNHEDEAALAKIKQEVNDWMVQFP
LY