Protein Info for Echvi_1032 in Echinicola vietnamensis KMM 6221, DSM 17526

Annotation: DNA polymerase I

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 937 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details PF02739: 5_3_exonuc_N" amino acids 8 to 175 (168 residues), 182.1 bits, see alignment E=1.7e-57 TIGR00593: DNA polymerase I" amino acids 9 to 937 (929 residues), 993.9 bits, see alignment E=4.6e-303 PF01367: 5_3_exonuc" amino acids 179 to 273 (95 residues), 110.3 bits, see alignment E=1.3e-35 PF01612: DNA_pol_A_exo1" amino acids 344 to 524 (181 residues), 113.9 bits, see alignment E=1.9e-36 PF22619: DNA_polI_exo1" amino acids 371 to 486 (116 residues), 33.7 bits, see alignment E=7.5e-12 PF00476: DNA_pol_A" amino acids 557 to 935 (379 residues), 553.5 bits, see alignment E=6e-170

Best Hits

KEGG orthology group: K02335, DNA polymerase I [EC: 2.7.7.7] (inferred from 64% identity to mtt:Ftrac_3236)

Predicted SEED Role

"DNA polymerase I (EC 2.7.7.7)" in subsystem DNA-replication or DNA Repair Base Excision (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0FW82 at UniProt or InterPro

Protein Sequence (937 amino acids)

>Echvi_1032 DNA polymerase I (Echinicola vietnamensis KMM 6221, DSM 17526)
MPSSNKKKLFLLDAMALIYRAHFAFSKNPRINSKGLNTGIMLGFTNTLLEVIEKQSPSHI
AVAFDTAAPTFRHEQFEAYKANRQEQPEDITVGIPWVKQIVDGFNIPILELDGYEADDII
GTIAKKAEYKDFEVYMMTPDKDYGQLVEEHIFLYKPAFMGNAVDVMGPKEVCAKWEIEDP
DQVRDILGLMGDAVDNIPGIPGIGEKTAKKLLKAYGTIEGLLEHVGDLKGKQKENVENFG
QQGLLSKSLATIKQDVPIEFNPEDLTYDGPDEEKLKALFAEMEFRTLTQRVFGEKMKKPA
VKVNEQLGLFTGPAEDAEEEEVEEANPIPVPDQLATVTSMVHAYHKVEGLAAIKELVEYL
EIQDEFCFDTETTDLDPNKAELVGLSFAYVPGEAFYIPVPADQEETKKLLEPLRGVFEDE
SITKIGQNIKYDALVLKNYGIALKGKLYDTMLAHYLIEPEGKHNMDWLAEHYLQYRPVSI
ETLIGKKGKSQGNMRDADVDKVVEYASEDADITLQLAQKLNPDLKERGLEKLFYEVETPL
IPVLTAMEYEGVKIDKESLAELSTALEKDITEIEARVYDLAGVKFNLASPKQLGEVLFVK
MELDPKAKKTKTGQYATGEEVLSKLAPKHEIARAILDYRELVKLKNTYVDTLPTLINPKT
DRIHTTYNQVVAATGRLSSINPNLQNIPIRTERGREIRKAFVPRDEAHVILAADYSQIEL
RIMAAFSKDESMMEAFREGRDIHSTTAAKIFQVPLEEVTSEMRRKAKTANFGIIYGISAF
GLSQRLNIPRGEAKEIIDAYFKEFPAVSQYMNDCIEKARKNEYVETILGRRRYLRDINSR
NATMRGYSERNAINAPIQGSAADMIKVAMIHVHQWMLEKQLKSKMILQVHDELVFDAHKD
EVDLLKKEIPKLMTKALEIEVPMQVEVGVGKDWLEAH