Protein Info for Echvi_0911 in Echinicola vietnamensis KMM 6221, DSM 17526

Annotation: isoleucyl-tRNA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1128 TIGR00392: isoleucine--tRNA ligase" amino acids 9 to 926 (918 residues), 976.6 bits, see alignment E=6.2e-298 PF00133: tRNA-synt_1" amino acids 19 to 706 (688 residues), 399.5 bits, see alignment E=3.1e-123 PF09334: tRNA-synt_1g" amino acids 51 to 193 (143 residues), 27.7 bits, see alignment E=2.4e-10 amino acids 654 to 717 (64 residues), 26.8 bits, see alignment 4.5e-10 PF08264: Anticodon_1" amino acids 759 to 915 (157 residues), 110 bits, see alignment E=1.9e-35 PF19302: DUF5915" amino acids 932 to 1128 (197 residues), 196.7 bits, see alignment E=6.1e-62

Best Hits

Swiss-Prot: 57% identical to SYI_BACTN: Isoleucine--tRNA ligase (ileS) from Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)

KEGG orthology group: K01870, isoleucyl-tRNA synthetase [EC: 6.1.1.5] (inferred from 57% identity to bth:BT_0806)

Predicted SEED Role

"Isoleucyl-tRNA synthetase (EC 6.1.1.5)" (EC 6.1.1.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0FTE6 at UniProt or InterPro

Protein Sequence (1128 amino acids)

>Echvi_0911 isoleucyl-tRNA synthetase (Echinicola vietnamensis KMM 6221, DSM 17526)
MKKYQEFKQVDYPGIGESILQYWQENKVFEKSVQNREGAETFTFFEGPPSANGTPGIHHV
MARTLKDIFCRYKTLKGYQVKRKGGWDTHGLPVELQVEKELGITKEDIGKKISVEEYNKK
CRETVMRFKDEWDDLTEKIGYWVDLDDPYITFDPKYIETLWSLLKRLYDKDLLYKGYTIQ
PYSPAAGTGLSSHELNQPGCYRDVKDTSITAQFKVKDRDNEYIIAWTTTPWTLPSNSALA
VGEKLDYVKVKTFNPYTHAPQTVILAKARMGAFFNKKAAELKLEDYKAGDKLIPYEVVEA
FKGADMLGMEYEPLFPIDGIKLPHPAYTVIPADYVTTEDGTGIVHLAKAFGADDFRTLVQ
AGVPGVFVKDEQDNEIPVVDKKGKFLPVVGEYLLAKMKEHEITAHKEYTADDFYVKNYLK
EDENAAEYKNTDVIISIILKNENKAFKVEKYEHSYPHCWRTDMPVLYYPLESWFIKTTAC
KDRLVELNKTINWKPEATGTGRFGNWLENLVDWNLSRSRFWGTPLPVWRNEDATETKCIG
SIAELTEEIEKSIAKGFMQESPFKGEEIDLHRPYVDDVILVDSQGNKMFREPDLIDVWFD
SGAMPYAQWHYPFENEEIFKANYPADYIAEGVDQTRGWFFTLHAIAVMLFDNVAFKNVIA
NGLVLDKNGNKMSKRLGNAVDPFKTLKEYGPDALRWYMLSNANPWDNLKFNLEGVAEVQR
RFFGTLQNTYNFFALYANLDAFTYDPAKTVAVSERPELDRWIVSKLQSLIKEVESSMDNY
DATRATRAIMNFTVDQLSNWYVRLARKRFWRGEMNQDKQAAYETLYECLMALSQLMSSFA
PFYSDWMFANLTEGAKEAGQEIPASVHLTDWKAADDGLINEDLEASMQLAQDISSLVHSL
RKKDKLKVRQPLQKILIPILNEKTRQQIQHVEELIKSEVNIKGVEYIDDASGILVKNAKP
NFPLLGKRFGPKMKLVAGAISKWGQEEINQLERDGSIEIEIDGEQARLAQEEVLITSQDI
PGWSVASANGITVALDVTLNDELKQEGIARDLVNRIQNLRKDMGLEVQDKITIKVAKLNE
QVDRALENFSAYIQSETQALSLSVNGDVEEGTVLDMDDFELKVKVEKV