Protein Info for Echvi_0877 in Echinicola vietnamensis KMM 6221, DSM 17526
Annotation: Formate-dependent nitrite reductase, periplasmic cytochrome c552 subunit
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 55% identical to NRFA_PARD8: Cytochrome c-552 (nrfA) from Parabacteroides distasonis (strain ATCC 8503 / DSM 20701 / CIP 104284 / JCM 5825 / NCTC 11152)
KEGG orthology group: K03385, formate-dependent nitrite reductase, periplasmic cytochrome c552 subunit [EC: 1.7.2.2] (inferred from 76% identity to mtt:Ftrac_2736)Predicted SEED Role
"Cytochrome c552 precursor (EC 1.7.2.2)" in subsystem Nitrate and nitrite ammonification or Soluble cytochromes and functionally related electron carriers (EC 1.7.2.2)
MetaCyc Pathways
- nitrate reduction IV (dissimilatory) (1/2 steps found)
- formate to nitrite electron transfer (1/3 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.7.2.2
Use Curated BLAST to search for 1.7.2.2
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See L0FWX9 at UniProt or InterPro
Protein Sequence (497 amino acids)
>Echvi_0877 Formate-dependent nitrite reductase, periplasmic cytochrome c552 subunit (Echinicola vietnamensis KMM 6221, DSM 17526) MKNWILFLITAVVVFLLAMLAYSIMDRKNEAKYAYQPKVDIGNIEPRDSIWGLNYPRQYQ SYMKTKDTSFHSMYNTSGHAAVLEEDPELVILWAGYGFSKEYNQPKGHAHAVEDIRKILR TGAPMEAGEGPMPSTCWTCKSPDVPRLMKEIGVTEFYSKKWSDLGAEVINPIGCADCHNP ETMNLTITRPALIEAFEAMGKNINESSYQQMRSLVCAQCHVEYYFAKDAPGKEGAQYLTF PWKDGMDVESMEAYYDELEFSDWVHPISKTPMLKAQHPDYELYSLGVHAKRGVSCADCHM PYKTEGGQKFTDHHIGSPLSNVENSCFVCHREKKEDLITDVFERQRMVKQGAQKLMRLVA MAHIEAGKAWELGAAEDQMKPIQEGIRHAQWRWDYAVASHGAAFHAPLPTNSIITSGTSI IEEARLQLARLLADLGHNKPVEMPDFKDTEALQAYVGMDMEKQRAQKEKFLEEVVPKWLE EGKKREAGYTIEMISNE