Protein Info for Echvi_0762 in Echinicola vietnamensis KMM 6221, DSM 17526

Annotation: NADH:ubiquinone oxidoreductase, Na(+)-translocating, F subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 434 signal peptide" amino acids 1 to 34 (34 residues), see Phobius details TIGR01941: NADH:ubiquinone oxidoreductase, Na(+)-translocating, F subunit" amino acids 5 to 434 (430 residues), 605.3 bits, see alignment E=2.6e-186 PF00111: Fer2" amino acids 47 to 117 (71 residues), 37.1 bits, see alignment E=3.6e-13 PF00970: FAD_binding_6" amino acids 214 to 283 (70 residues), 27.8 bits, see alignment E=4e-10 PF00175: NAD_binding_1" amino acids 294 to 413 (120 residues), 37.5 bits, see alignment E=4.8e-13

Best Hits

Swiss-Prot: 53% identical to NQRF_VIBAN: Na(+)-translocating NADH-quinone reductase subunit F (nqrF) from Vibrio anguillarum

KEGG orthology group: K00351, Na+-transporting NADH:ubiquinone oxidoreductase subunit F [EC: 1.6.5.-] (inferred from 81% identity to mtt:Ftrac_1690)

MetaCyc: 53% identical to Na(+)-translocating NADH-quinone reductase subunit F (Vibrio cholerae)
TRANS-RXN-214 [EC: 7.2.1.1]

Predicted SEED Role

"Na(+)-translocating NADH-quinone reductase subunit F (EC 1.6.5.-)" in subsystem Na(+)-translocating NADH-quinone oxidoreductase and rnf-like group of electron transport complexes (EC 1.6.5.-)

Isozymes

Compare fitness of predicted isozymes for: 1.6.5.-

Use Curated BLAST to search for 1.6.5.- or 7.2.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0FUS5 at UniProt or InterPro

Protein Sequence (434 amino acids)

>Echvi_0762 NADH:ubiquinone oxidoreductase, Na(+)-translocating, F subunit (Echinicola vietnamensis KMM 6221, DSM 17526)
MSSVIITSIVAFTLIILLLVFILLFAQSKLVASGDVNIIINGDESNPVVASAGSTLLSTL
GGQKIFLPSACGGGGTCAMCKCTVEEGGGEVLPTEVGHLSRAEQQSNVRLSCQVKVKQDM
RIRIPEEIFGIKKWECEVLSNYNVSTFIKEFKVKLPEGETLDFESGGYIQIDVPEITVNF
KDIDITPHPELEHPEDVYQSDWDKFGLWDLVMKNDEELFRAYSMANHPAEGNIIMLTIRI
ATPPWDRANNKWMDVNPGVCSSYVFSRKPGDKVTISGPYGEFHINPTEREMIYIGGGAGM
APLRSHIFHLFHTEKTNRKVSYWYGGRSKKELFYVPHFRDIEKDFDNFRFYIGLSEPLPE
DNWKIKKSLEDREGDGYVGFIHQVLYDNYLKDHPEPDEVEYYLCGPPLMNSAVLKLLDDM
GVPKENIRFDDFGG