Protein Info for Echvi_0758 in Echinicola vietnamensis KMM 6221, DSM 17526

Annotation: N-acetylmuramic acid 6-phosphate etherase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 268 TIGR00274: N-acetylmuramic acid 6-phosphate etherase" amino acids 3 to 255 (253 residues), 332.4 bits, see alignment E=1e-103 PF01380: SIS" amino acids 124 to 203 (80 residues), 30.8 bits, see alignment E=2.3e-11 PF20741: GKRP-like_C" amino acids 217 to 254 (38 residues), 30.3 bits, see alignment 5.1e-11

Best Hits

Swiss-Prot: 68% identical to MURQ_GRAFK: N-acetylmuramic acid 6-phosphate etherase (murQ) from Gramella forsetii (strain KT0803)

KEGG orthology group: K07106, N-acetylmuramic acid 6-phosphate etherase [EC: 4.2.-.-] (inferred from 66% identity to psn:Pedsa_1860)

MetaCyc: 52% identical to N-acetylmuramic acid 6-phosphate etherase (Escherichia coli K-12 substr. MG1655)
RXN0-4641 [EC: 4.2.1.126]

Predicted SEED Role

"N-acetylmuramic acid 6-phosphate etherase"

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.2.-.-, 4.2.1.126

Use Curated BLAST to search for 4.2.-.- or 4.2.1.126

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0FVK3 at UniProt or InterPro

Protein Sequence (268 amino acids)

>Echvi_0758 N-acetylmuramic acid 6-phosphate etherase (Echinicola vietnamensis KMM 6221, DSM 17526)
MKTTESSSHYDNLENMSIAELISNMNKEDQTVPVAVSKALPAIEKLIEKIVDRMAQGGRL
FYIGAGTSGRLGILDASECPPTYGVSHDMVIGLIAGGDTAIRKAVERAEDDPDQSWQDLK
ANGIQELDTVIGIAASGTTPYVIGGVKTAREKGLLTGCITCNEGSPLAKVAEYPVEVVVG
PEFVTGSTRMKSGTAQKLVLNMISTSVMIKLGKVKGNKMVNMQLSNQKLVERGTRMIMEA
TGLAEEQAKERLLKFGSVKDALDDWKQE