Protein Info for Echvi_0693 in Echinicola vietnamensis KMM 6221, DSM 17526

Annotation: D-alanine--D-alanine ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 329 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details TIGR01205: D-alanine--D-alanine ligase" amino acids 4 to 325 (322 residues), 283.7 bits, see alignment E=8.1e-89 PF01820: Dala_Dala_lig_N" amino acids 4 to 106 (103 residues), 69.8 bits, see alignment E=9.3e-23 PF02786: CPSase_L_D2" amino acids 122 to 294 (173 residues), 38.9 bits, see alignment E=2e-13 PF02222: ATP-grasp" amino acids 131 to 293 (163 residues), 33.2 bits, see alignment E=1.2e-11 PF07478: Dala_Dala_lig_C" amino acids 140 to 324 (185 residues), 169.8 bits, see alignment E=1.8e-53 PF02655: ATP-grasp_3" amino acids 142 to 294 (153 residues), 35 bits, see alignment E=4.7e-12 PF13535: ATP-grasp_4" amino acids 155 to 295 (141 residues), 35.5 bits, see alignment E=2.3e-12

Best Hits

KEGG orthology group: K01921, D-alanine-D-alanine ligase [EC: 6.3.2.4] (inferred from 59% identity to phe:Phep_1847)

Predicted SEED Role

"D-alanine--D-alanine ligase (EC 6.3.2.4)" in subsystem Peptidoglycan Biosynthesis (EC 6.3.2.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0FWD0 at UniProt or InterPro

Protein Sequence (329 amino acids)

>Echvi_0693 D-alanine--D-alanine ligase (Echinicola vietnamensis KMM 6221, DSM 17526)
MKKKIALVTGGFTGESVVSLKSAAVVETQIDRERYDVYKIIIEKENWFYAAEDGTPSLVD
LNDFSIQVDGKKVTFDGVFNILHGSPGEDGKLAGYFDMVGLPYTTCDALTSAITMNKGYT
KAIVQDIEGLEVANSLQLFKNSPKNTQRVIDELTLPYFVKPNNGGSSIGMSKVKSEGEVQ
EALDKAFAEDNQVLIEEFVSGREFSIGMYMVNGEVIVLPATEIVSSKEFFDFEAKYTAGV
TEEITPGRMSEEEVNRVREVARKVYLKLNCKGAVRMDYFLEKETGKIFFIEINTVPGQTE
TSLISQQVRAIGKTVKEFYTEIIEEMWKQ