Protein Info for Echvi_0642 in Echinicola vietnamensis KMM 6221, DSM 17526

Annotation: pseudouridine synthase, RluA family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 364 TIGR00005: pseudouridine synthase, RluA family" amino acids 47 to 354 (308 residues), 309.1 bits, see alignment E=1.6e-96 PF01479: S4" amino acids 51 to 96 (46 residues), 38 bits, see alignment 1.1e-13 PF00849: PseudoU_synth_2" amino acids 124 to 281 (158 residues), 124.6 bits, see alignment E=4.1e-40

Best Hits

KEGG orthology group: K06180, ribosomal large subunit pseudouridine synthase D [EC: 5.4.99.12] (inferred from 66% identity to mtt:Ftrac_1448)

Predicted SEED Role

"Ribosomal large subunit pseudouridine synthase D (EC 4.2.1.70)" in subsystem Ribosome biogenesis bacterial (EC 4.2.1.70)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.70, 5.4.99.12

Use Curated BLAST to search for 4.2.1.70 or 5.4.99.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0FW74 at UniProt or InterPro

Protein Sequence (364 amino acids)

>Echvi_0642 pseudouridine synthase, RluA family (Echinicola vietnamensis KMM 6221, DSM 17526)
MAVDPYFCRMNDEMDDELFDFDGAAEDDQTDGASGYEHLRIAVDKGQAATRVDKFLTDKV
ANVTRNKVQQAIDNGQVKINGSNTKANYKIKPGDVITVVLEMEPRETEVLPEKLDLDIVH
EDDHLLVVNKPAGMVVHPAYGNWTGTLVNGLVYHFSQLPEMPGNSGRPGLVHRIDKDTSG
LLVIAKSEQAMTGLAKQFFDHSIERTYIALVWGEPQEDAGTIDAHVGRSARDRKVMDVFP
EGDQGKNAVTHWKVLKRLRYVSLVQCNLETGRTHQIRAHMKYLGHPLFNDAVYGGDKIRK
GTQFSKYKAFVNNCFKLLPRQALHAKSLGFSHPISQDFLQFTSNLPEDMQSVLERWENYV
NFEG