Protein Info for Echvi_0564 in Echinicola vietnamensis KMM 6221, DSM 17526

Annotation: ribosomal protein S12 methylthiotransferase RimO

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 437 PF00919: UPF0004" amino acids 10 to 111 (102 residues), 89.7 bits, see alignment E=2.1e-29 TIGR00089: radical SAM methylthiotransferase, MiaB/RimO family" amino acids 10 to 433 (424 residues), 407.8 bits, see alignment E=5.2e-126 TIGR01125: ribosomal protein S12 methylthiotransferase RimO" amino acids 10 to 433 (424 residues), 489.9 bits, see alignment E=7.7e-151 PF04055: Radical_SAM" amino acids 146 to 317 (172 residues), 81.5 bits, see alignment E=1.6e-26 PF01938: TRAM" amino acids 371 to 436 (66 residues), 26.9 bits, see alignment E=7.1e-10 PF18693: TRAM_2" amino acids 373 to 436 (64 residues), 70.3 bits, see alignment E=2.5e-23

Best Hits

Swiss-Prot: 69% identical to RIMO_CYTH3: Ribosomal protein S12 methylthiotransferase RimO (rimO) from Cytophaga hutchinsonii (strain ATCC 33406 / NCIMB 9469)

KEGG orthology group: K14441, ribosomal protein S12 methylthiotransferase [EC: 2.-.-.-] (inferred from 74% identity to mtt:Ftrac_1929)

Predicted SEED Role

"Ribosomal protein S12p Asp88 (E. coli) methylthiotransferase" in subsystem Ribosomal protein S12p Asp methylthiotransferase

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.-.-.-

Use Curated BLAST to search for 2.-.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0FU65 at UniProt or InterPro

Protein Sequence (437 amino acids)

>Echvi_0564 ribosomal protein S12 methylthiotransferase RimO (Echinicola vietnamensis KMM 6221, DSM 17526)
MKARTLKKDKVNIVTMGCSKNLVDSEVMLTQLKGNGIEVSHESLQQDNNVIIINTCGFID
NAKQESIDTILQYVDAKEKGLVEKVYVTGCLSQRYKDDLEKEIPQVDAFFGTRDLPALLK
KFKADYKHELVGERLLTHPSHYAYMKISEGCDRPCSFCAIPLMRGGHVSKPIEELVKEAE
HKAANGTKELLLIAQDSTYYGLDLYKKRRLADLMKALADVNGIDWVRLHYAYPTGFPMDV
IDVMAEHPNICNYLDIPLQHGSSDVLKVMRRGTSREKQEELIHRIREKIPGIGIRTTLIA
GHPGEGEKEFQEMVDFVERMKFERLGVFTYSHEEDTHAFTMNDDVPDEEKQARANHLMEV
QEQISFDLNQEKVGQTFKVLVDKKENGYFVGRTEFDSVEVDNEVLIDASKHYCRVGDFVQ
VKVNDATEFDLYGDVVD