Protein Info for Echvi_0485 in Echinicola vietnamensis KMM 6221, DSM 17526

Annotation: Beta-galactosidase/beta-glucuronidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1038 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details PF02837: Glyco_hydro_2_N" amino acids 65 to 234 (170 residues), 171.5 bits, see alignment E=4.7e-54 PF22666: Glyco_hydro_2_N2" amino acids 139 to 205 (67 residues), 40.5 bits, see alignment 9.2e-14 PF00703: Glyco_hydro_2" amino acids 238 to 345 (108 residues), 56.3 bits, see alignment E=1.5e-18 PF02836: Glyco_hydro_2_C" amino acids 347 to 637 (291 residues), 333.8 bits, see alignment E=2.7e-103 PF16353: LacZ_4" amino acids 648 to 731 (84 residues), 77.7 bits, see alignment E=2e-25 PF02929: Bgal_small_N" amino acids 762 to 1034 (273 residues), 233.3 bits, see alignment E=1.1e-72

Best Hits

KEGG orthology group: None (inferred from 62% identity to zpr:ZPR_0911)

Predicted SEED Role

"Beta-galactosidase (EC 3.2.1.23)" in subsystem Galactosylceramide and Sulfatide metabolism or Lactose and Galactose Uptake and Utilization or Lactose utilization (EC 3.2.1.23)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.23

Use Curated BLAST to search for 3.2.1.23

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0FS56 at UniProt or InterPro

Protein Sequence (1038 amino acids)

>Echvi_0485 Beta-galactosidase/beta-glucuronidase (Echinicola vietnamensis KMM 6221, DSM 17526)
MQFKKLWMTGALVAALGGLLHAQSQNEWEDPTAVDRNKEAARAYFITYPSEEKALLGNRT
TNESFKTLDGLWKFSLVKRPQDRPTDFFEPTFKDEDWDDITVPSNWELEGYDMPVYTNVA
YPFPADPPLVDNQYNPVGTYRRTFSIPSQWDNQEVILHFGSISGYATVYVNGEEVGMTKA
AKTPAEFVITDYLKTGENTLAVQVFRWHDGSYLEDQDFWRLSGIERSVFLQAVPKLTIWD
FFVKSGLDDRYKNGVLEAAIQLRAFEGSDVQGGELSFELQDEDGKQVYSDTKAVSNGDQE
VKFSKTIGNVNKWSAEEPYLYQYTISLKDSRGRTLAAVSKKTGFRKVEIKDAQLMVNGQS
VLVKGVNRHEHHGVKGHVPDEEIMLRDIQLMKQNNINAVRMSHYPHSPRWYELCDEYGLY
VVDEANIETHGMGAEWQGRFKKDRHPAYLEAWAPAHLDRIHRLVERDKNHPSIIIWSMGN
ECGNGPVFYEAYNWMKERDDSRLVQFEQAGENEDTDIVCPMYPSIRHMQEYADATDKTRP
FIMCEYAHSMGNSTGNFQEYWDIILDSPHMQGGFIWDWVDQGLLAKDDNGKEFWAYGGDL
GGYFFQNDENFCANGLVTADRKPHPALHEVKKVYQDILFDYSPEKGLHVQNLFDFTNLDQ
YAFKWEWVEEGEVVKTGDFDVDLSADEEKYVQLNLPSVGDAETFLNVYAYTKNTEALVPA
GHEVAREQFALNEGYYFDHLEAVTGNLQVEQTEDLLTFATDKVTGAFDLKRGNFRKYTLK
DGEPWMVRSLPSPYFWRAPIDNDFGNHMPSRLGVWRSAHLGQKVLDVQVGEKSDEGIQIT
VNYELTNINVPYTVTYQIQSDGAVKVTAAMDLEGRDLPELPRYGMRMELPGQYGNLAYYG
RGPWENYSDRKHSSFIGQYNDQVENQFYWDYVRPQESGNKTDVRWLTLRNDKGQGIQIQG
IQPLSFSALDVSVEDLDPGLTKKQQHPTDIKPKNTVYLHIDWKQRGLGGDTSWGAYPHKP
YRLEDDHYEYSYVIRLVE