Protein Info for Echvi_0292 in Echinicola vietnamensis KMM 6221, DSM 17526

Annotation: Superfamily I DNA and RNA helicases

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 752 PF00580: UvrD-helicase" amino acids 7 to 276 (270 residues), 300.5 bits, see alignment E=5e-93 PF13245: AAA_19" amino acids 11 to 261 (251 residues), 81.5 bits, see alignment E=2.2e-26 PF13361: UvrD_C" amino acids 281 to 617 (337 residues), 267.4 bits, see alignment E=9e-83 PF13538: UvrD_C_2" amino acids 556 to 614 (59 residues), 37.1 bits, see alignment 7.9e-13 PF21196: PcrA_UvrD_tudor" amino acids 704 to 746 (43 residues), 30.4 bits, see alignment 9.7e-11

Best Hits

KEGG orthology group: K03657, DNA helicase II / ATP-dependent DNA helicase PcrA [EC: 3.6.4.12] (inferred from 73% identity to mtt:Ftrac_0837)

Predicted SEED Role

"ATP-dependent DNA helicase UvrD/PcrA" in subsystem DNA repair, bacterial UvrD and related helicases

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.12

Use Curated BLAST to search for 3.6.4.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0FU95 at UniProt or InterPro

Protein Sequence (752 amino acids)

>Echvi_0292 Superfamily I DNA and RNA helicases (Echinicola vietnamensis KMM 6221, DSM 17526)
MDYLEGLNPPQREAVEHTEGPLMIIAGAGSGKTRVLTFRIAHLIRAKNVDPFQILALTFT
NKAASEMRNRIERLIGLEARNTWMGTFHSIFAKILRVESEKLGFPSNFTIYDTDDSKSLI
KSIVKEKRLDDKVYKPNTVLSRISGAKNRLISWENYINDPYIKADDEAAMKPRMGEIYRA
YQKRLFKSSAMDFDDLLFNTNVLFRDHPDVLNKYQQRFRYVLVDEFQDTNLSQYLITKKL
AAVHQNICVVGDDAQSIYAFRGADIQNILNFEKDYPDLHVVKLEQNYRSTKNIVEAANSI
IEKNKAQLKKNVWTQNGNGDLIELIKSASDNEEGRMVASTIFEEKNNKKLQNSDFAILYR
TNSQSRALEEALRKMNISYKIVGGLSFYQRKEIKDLMAYFRYVVNQDDEEAFKRIINYPK
RGIGLSSVEKMMVAAYEHDIPLWQVVTNSSSFLSGRASNSVSDFATMIKSFQMEVERKDA
YEAASTIAKQSGLLRELYEDKTIEGLNRYENVQELLNAIKEYVDNPENEDKNLGAFLQEI
ALLTDNDRDKDEMDAVTLMTIHSSKGLEFRQVFVVGLEEDLFPSQMMMQSREDLEEERRL
FYVATTRAMEKLYLSYAITRYRFGRLLNCEPSRFLEEIDPSCIRVTKRRGQQPTGSHFGR
NNDSEGRSGFVGLKTPSVRKNTTAKVHTPSPDFKPSNTSNLAAGMKVEHPKFGFGKVLKV
EVEGLNKKATIGFDNFGEKTLLLSFAKLRIVE