Protein Info for Echvi_0146 in Echinicola vietnamensis KMM 6221, DSM 17526

Annotation: Nucleoside-diphosphate-sugar epimerases

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 325 PF04321: RmlD_sub_bind" amino acids 10 to 167 (158 residues), 54 bits, see alignment E=4.1e-18 PF01370: Epimerase" amino acids 11 to 249 (239 residues), 107.5 bits, see alignment E=2.2e-34 PF02719: Polysacc_synt_2" amino acids 11 to 121 (111 residues), 34.5 bits, see alignment E=4.1e-12 PF16363: GDP_Man_Dehyd" amino acids 12 to 175 (164 residues), 68.2 bits, see alignment E=2.7e-22 PF01073: 3Beta_HSD" amino acids 12 to 136 (125 residues), 29.3 bits, see alignment E=1.4e-10 PF07993: NAD_binding_4" amino acids 72 to 227 (156 residues), 27.9 bits, see alignment E=4e-10

Best Hits

Swiss-Prot: 51% identical to Y2164_STAS1: Uncharacterized epimerase/dehydratase SSP2164 (SSP2164) from Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229)

KEGG orthology group: None (inferred from 72% identity to mtt:Ftrac_2956)

Predicted SEED Role

"L-threonine 3-dehydrogenase (EC 1.1.1.103)" in subsystem Glycine Biosynthesis or Threonine degradation (EC 1.1.1.103)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.103

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0FTT1 at UniProt or InterPro

Protein Sequence (325 amino acids)

>Echvi_0146 Nucleoside-diphosphate-sugar epimerases (Echinicola vietnamensis KMM 6221, DSM 17526)
MARLIEIMETILITGAAGQLGSELTLALANMYGGDRIIATDINEASAYKFDYCRFMALDV
MDRKRMTEIVKAEKVTQIYHLAAILSATSEKNPIFAWNLNMESLLSILEIAREQKLNKIY
WPSSIAVFGPNTPMQHTPQDCIMDPNTVYGISKQAGERWCAYYFEKFGVDVRSLRYPGLI
GYKSMPGGGTTDYAVDIFHKAIEGEDFECFLEEDTYLPMMYMDDAIKATLDLMHAPAAQI
KVRSSYNLGGISFSPKEIYECIKQHYPNFNITYKPDFRQQIADTWPDSIDDSAAQNDWGW
QHSYGLEEMTKDILANLPAYLVNLK