Protein Info for Echvi_0145 in Echinicola vietnamensis KMM 6221, DSM 17526

Annotation: 2-amino-3-ketobutyrate coenzyme A ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 397 transmembrane" amino acids 272 to 291 (20 residues), see Phobius details TIGR01822: glycine C-acetyltransferase" amino acids 6 to 397 (392 residues), 715.9 bits, see alignment E=6.1e-220 PF00155: Aminotran_1_2" amino acids 43 to 387 (345 residues), 232.7 bits, see alignment E=3.9e-73

Best Hits

Swiss-Prot: 62% identical to KBL_SHIFL: 2-amino-3-ketobutyrate coenzyme A ligase (kbl) from Shigella flexneri

KEGG orthology group: K00639, glycine C-acetyltransferase [EC: 2.3.1.29] (inferred from 79% identity to shg:Sph21_1459)

MetaCyc: 62% identical to 2-amino-3-ketobutyrate CoA ligase (Escherichia coli K-12 substr. MG1655)
Glycine C-acetyltransferase. [EC: 2.3.1.29]

Predicted SEED Role

"2-amino-3-ketobutyrate coenzyme A ligase (EC 2.3.1.29)" in subsystem Glycine Biosynthesis or Glycine and Serine Utilization (EC 2.3.1.29)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.29

Use Curated BLAST to search for 2.3.1.29

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0FT22 at UniProt or InterPro

Protein Sequence (397 amino acids)

>Echvi_0145 2-amino-3-ketobutyrate coenzyme A ligase (Echinicola vietnamensis KMM 6221, DSM 17526)
MYESLKPKLEKELKEISDAGLYKSERIITSPQGAEISTSEGKQVLNFCANNYLGLSSHPK
VIEAAKNAIDTHGYGMSSVRFICGTQDIHKELERKISEFLGTEDTILYAAAFDANGGVFE
PILGPEDAIISDALNHASIIDGVRLCKAMRFRYKHNDMEDLETQLKEANAKGAKQKIIVT
DGAFSMDGTIAQMDKIVALAEQYDALVMSDECHSTGFIGKTGRGVHELKGVMGKMDIITG
TLGKALGGASGGFTSGRKEIIDILRQRSRPYLFSNTLAPAITGASIAVFDLLSETTELRD
KLEDNTTYFREKMTAAGFDIKPGVHPIVPIMLYDAVLSQQMAEKLLERGVYVIGFYYPVV
PKGQARIRVQISAAHDRKHLDAAIEAFTEVGKELGVI