Protein Info for EX28DRAFT_4490 in Enterobacter asburiae PDN3

Annotation: Lysophospholipase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 330 PF12146: Hydrolase_4" amino acids 52 to 312 (261 residues), 177.2 bits, see alignment E=5.4e-56 PF00561: Abhydrolase_1" amino acids 56 to 312 (257 residues), 120.5 bits, see alignment E=1.5e-38 PF12697: Abhydrolase_6" amino acids 70 to 283 (214 residues), 37.3 bits, see alignment E=7.6e-13

Best Hits

Swiss-Prot: 83% identical to PLDB_ECOL6: Lysophospholipase L2 (pldB) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K01048, lysophospholipase [EC: 3.1.1.5] (inferred from 95% identity to enc:ECL_04970)

MetaCyc: 83% identical to lysophospholipase L2 (Escherichia coli K-12 substr. MG1655)
Lysophospholipase. [EC: 3.1.1.5]; 3.1.1.5 [EC: 3.1.1.5]; RXN0-7122 [EC: 3.1.1.5]

Predicted SEED Role

"Lysophospholipase L2 (EC 3.1.1.5)" in subsystem Synechocystis experimental or Triacylglycerol metabolism (EC 3.1.1.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.1.5

Use Curated BLAST to search for 3.1.1.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (330 amino acids)

>EX28DRAFT_4490 Lysophospholipase (Enterobacter asburiae PDN3)
MFQQKKDWGTRENAFAAFSMGPLTDFWRQREEDEFTGVGGIPVRFVRFRNEKNDRVIVVC
PGRIESYVKYAELAYDLVHMGFDVLIIDHRGQGLSGRMLPDTHRGHVDRFSDYVDDFAAF
WQQDVQPGPWRKRYILAHSMGGAISTLFLQRYAHECDAIALTAPMYGIVIRFPDWMVRHL
LDWAEGHQRIREGYAIGTGRWRALPFAINVLTHSRQRYRRNLRFYADEPRLRVGGPTWHW
VREGILAGEQVLAGVGDDDTPTLLIQAEEERVVDNRMHDRYCELRAAAGHPCEGGKPLVI
NGAFHEILFEKDAMRSVALNAIVEFFNRHN