Protein Info for EX28DRAFT_4429 in Enterobacter asburiae PDN3

Annotation: TonB-dependent vitamin B12 receptor

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 615 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details TIGR01779: TonB-dependent vitamin B12 receptor" amino acids 2 to 615 (614 residues), 812.5 bits, see alignment E=1.3e-248 PF07715: Plug" amino acids 39 to 145 (107 residues), 100.6 bits, see alignment E=7.3e-33 PF00593: TonB_dep_Rec" amino acids 183 to 589 (407 residues), 137.5 bits, see alignment E=1.3e-43

Best Hits

Swiss-Prot: 93% identical to BTUB_ENT38: Vitamin B12 transporter BtuB (btuB) from Enterobacter sp. (strain 638)

KEGG orthology group: K02014, iron complex outermembrane recepter protein (inferred from 93% identity to ent:Ent638_4020)

MetaCyc: 78% identical to cobalamin outer membrane transporter (Escherichia coli K-12 substr. MG1655)
RXN0-1565; TRANS-RXN0-283

Predicted SEED Role

"Outer membrane vitamin B12 receptor BtuB" in subsystem Coenzyme B12 biosynthesis

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (615 amino acids)

>EX28DRAFT_4429 TonB-dependent vitamin B12 receptor (Enterobacter asburiae PDN3)
MIKKVSLLTALSVTAFSGWAQDSADSLVVTANRFEQPEKTILAATSVVTRADIDRWQSTS
VLDVMRRLPGVDTAQNGGMGQLSSLFIRGTNSSHVLILVDGIRLNQAGVTGSSDLSQFPI
SLVQRIEYVRGPRSAVYGSDAIGGVVNIITTRAKDGTTLNAGVGSHGYQNYGGSTQQTLG
DSTRVTLAGDYTYTKGFDVVADGNNGGLAQTDRDGFMNKTLYGALDHAFSEQWSGFVRGF
GYSNRTAYDGYYSSFTPDVLVDTRQLYSQTWDAGLRFNDDIFHSQLLTSYSHSKDYNYDP
NLGRYDSTATLDEIKQYNVQWLNSVDVGHGNIGAGVDWQKQSTEPGTNYVTNGYDLRNTG
VYLTGLQQFGDFILEGAVRSDDNSQFGRHGTWQSSAAWEFVEGYRFVASYGTAYKAPNLG
QLYGFYGNDHLDPEESKQWEGAFEGLTAGVSWRVSGYRNDVDNLIDFDNNLQQYYNVGKA
RIKGVEATASFDTGPLTHTVGYDYVDARNAATNELLDRRAKQQVKYQLDTQIYDFDWSLT
YHYLGTRYDTDFGAYPSEKVKMGGVSLWDVAVSYPVTSHLTVRGKIANLFDKDYETVYGY
QTAGREYTLSGSYTF