Protein Info for EX28DRAFT_4391 in Enterobacter asburiae PDN3

Annotation: sulfate/thiosulfate-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 329 signal peptide" amino acids 1 to 20 (20 residues), see Phobius details TIGR00971: sulfate ABC transporter, sulfate-binding protein" amino acids 12 to 326 (315 residues), 628 bits, see alignment E=1.4e-193 PF01547: SBP_bac_1" amino acids 33 to 272 (240 residues), 74.4 bits, see alignment E=3.2e-24 PF13531: SBP_bac_11" amino acids 41 to 280 (240 residues), 145.8 bits, see alignment E=3.6e-46 PF13416: SBP_bac_8" amino acids 87 to 287 (201 residues), 40.1 bits, see alignment E=7.9e-14 PF13343: SBP_bac_6" amino acids 128 to 291 (164 residues), 40.3 bits, see alignment E=5.2e-14

Best Hits

Swiss-Prot: 93% identical to SUBI_SALTY: Sulfate-binding protein (sbp) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K02048, sulfate transport system substrate-binding protein (inferred from 98% identity to enc:ECL_05060)

MetaCyc: 93% identical to sulfate/thiosulfate ABC transporter periplasmic binding protein Sbp (Escherichia coli K-12 substr. MG1655)
ABC-19-RXN [EC: 7.3.2.5]; ABC-7-RXN [EC: 7.3.2.5, 7.3.2.3]; 7.3.2.3 [EC: 7.3.2.5, 7.3.2.3]; TRANS-RXN0-478 [EC: 7.3.2.5, 7.3.2.3]; TRANS-RXN0-479 [EC: 7.3.2.5, 7.3.2.3]

Predicted SEED Role

"Sulfate-binding protein Sbp"

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.3.2.3 or 7.3.2.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (329 amino acids)

>EX28DRAFT_4391 sulfate/thiosulfate-binding protein (Enterobacter asburiae PDN3)
MNKWGVGLTLLLASTSVLAKDIQLLNVSYDPTRELYEQYNKAFAAHWKQETGDNVVVRQS
HGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKNWIKRLPDNSAPYTSTIVFLV
RKGNPKQIKDWNDLIKPGVSVITPNPKSSGGARWNYLGAWGYALHHNNGDQAKAQEFVKA
LYKNVEVLDSGARGATNTFVERGIGDVLIAWENEALLATNELGKDKFEIVTPSESILAEP
TVSVVDKVVEKKDTKAVAEAYLKYLYSPEGQEIAAKNFYRPRDPAVAKKYESVFPKLKLF
TIDDEFGGWTKAQKEHFSNGGTFDQISQR