Protein Info for EX28DRAFT_4343 in Enterobacter asburiae PDN3

Annotation: DNA polymerase I (EC 2.7.7.7)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 930 transmembrane" amino acids 763 to 776 (14 residues), see Phobius details PF02739: 5_3_exonuc_N" amino acids 9 to 170 (162 residues), 196.6 bits, see alignment E=4.8e-62 TIGR00593: DNA polymerase I" amino acids 9 to 930 (922 residues), 956.4 bits, see alignment E=9.6e-292 PF01367: 5_3_exonuc" amino acids 171 to 271 (101 residues), 107.6 bits, see alignment E=7.9e-35 PF01612: DNA_pol_A_exo1" amino acids 332 to 519 (188 residues), 137.7 bits, see alignment E=7.3e-44 PF00476: DNA_pol_A" amino acids 553 to 927 (375 residues), 563.3 bits, see alignment E=5.2e-173

Best Hits

Swiss-Prot: 91% identical to DPO1_SALTY: DNA polymerase I (polA) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K02335, DNA polymerase I [EC: 2.7.7.7] (inferred from 90% identity to cko:CKO_03151)

MetaCyc: 91% identical to DNA polymerase I (Escherichia coli K-12 substr. MG1655)
3.1.11.-; 3.1.11.-; DNA-directed DNA polymerase. [EC: 2.7.7.7]

Predicted SEED Role

"DNA polymerase I (EC 2.7.7.7)" in subsystem DNA-replication or DNA Repair Base Excision (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (930 amino acids)

>EX28DRAFT_4343 DNA polymerase I (EC 2.7.7.7) (Enterobacter asburiae PDN3)
MVQIPENPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLNMLRSLILQYQPTHAAV
VFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHTMVKAMGLPLLAVSGVEADDVIGT
LAREAEKMGRPVLISTGDKDMAQLVTPGITLINTMTNTILGPEEVVTKYGVPPELIIDFL
ALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAESDKIAGLSFRGAKTMAGKLEENK
EVAYLSYKLATIKTDVELELGCEQLEVQQPSADDLLSLFKKYEFKRWITDVEAGKWLQAK
GAKPAAKPKETIVVDAEEQAEEEAVALSFDNYETILEESQLIAWIEKLKKAPVFAFDTET
DSLDNISANMVGLSFATEPGIAAYVPVAHDYLDAPEQISRERVLELLKPILEDEKALKVG
QNLKYDRGILQNYGIELRGIAFDTMLESYILDSVAGRHDMDSLSDRWLKHKTITFEEIAG
KGKNQLTFNQIALEEAGRYAAEDADVTLQLHLKMWPKLQKHEGPLNVFQHIEMPLVPVLS
RIERNGVKIDPTVLHNHSGELAQRLTELEQKAHELAGEPFNLSSPKQLQTILFEKQGIKP
LKKTPGGAPSTSEEVLEELALDYPLPKVILQYRGLAKLKSTYTDKLPLMINPKTGRVHTS
YHQAVAATGRLSSTDPNLQNIPVRNEEGRRIRQAFIAPEDYLIVSADYSQIELRIMAHLS
RDKGLLTAFAEGKDIHRATAAEVFGLPLDSVTNEQRRSAKAINFGLIYGMSAFGLSRQLN
IPRKESQKYMDLYFERYPGVLEYMERTRAQAKEKGYVETLDGRRLYLPDIKSSNAARRAG
AERAAINAPMQGTAADIIKRAMIAVDAWLEEEKPRVKMIMQVHDELVFEVHKDDLDAVSK
KIHELMESSMTLDVPLLVEVGSGENWDQAH