Protein Info for EX28DRAFT_4328 in Enterobacter asburiae PDN3

Annotation: Isocitrate dehydrogenase kinase/phosphatase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 583 PF20423: AceK_regulatory" amino acids 10 to 307 (298 residues), 368.4 bits, see alignment E=2.7e-114 PF06315: AceK_kinase" amino acids 310 to 564 (255 residues), 402.1 bits, see alignment E=1.1e-124

Best Hits

Swiss-Prot: 90% identical to ACEK_ENT38: Isocitrate dehydrogenase kinase/phosphatase (aceK) from Enterobacter sp. (strain 638)

KEGG orthology group: K00906, isocitrate dehydrogenase kinase/phosphatase [EC: 2.7.11.5 3.1.3.-] (inferred from 95% identity to enc:ECL_00268)

MetaCyc: 88% identical to isocitrate dehydrogenase kinase/phosphatase (Escherichia coli K-12 substr. MG1655)
3.1.3.-; [Isocitrate dehydrogenase (NADP(+))] kinase. [EC: 2.7.11.5]

Predicted SEED Role

"Isocitrate dehydrogenase phosphatase (EC 2.7.11.5)/kinase (EC 3.1.3.-)" (EC 2.7.11.5, EC 3.1.3.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.3.-

Use Curated BLAST to search for 2.7.11.5 or 3.1.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (583 amino acids)

>EX28DRAFT_4328 Isocitrate dehydrogenase kinase/phosphatase (Enterobacter asburiae PDN3)
MSRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEHADWHAVQQAMKQRIHLYDHHVGL
VVEQLRCITDGKSPDADFLLRVKEHYTHLLPDYPRFEIAESFFNSVYCRLFDHRSLSPER
LFIFSSQPERRFRTIPRPLAKNFFPDRGWEKLLHRVLTDLPLRLPWENKTRDIDYIHTHL
NETFGAEVLSHSHLQVANELFYRNKAAWLVGKLVTPTAIVPFLLPIHRTDDGELFVDTCL
TTGAEASIVFGFARSYFMVYAPLPAALVEWLREILPGKTTAELYMAIGCQKHAKTESYRE
YLRYVTTADEQFIEAPGIRGMVMLVFTLPGFDRVFKVIKDRFAPQKEMTAAHVRACYQLV
KEHDRVGRMADTQEFENFVLDKQQIDPALMALLMQEAPAKITDLGDKIVISHLYIERRMV
PLNIWLEQSEGQALRDAIEEYGNAIRQLAAANIFPGDMLFKNFGVTRHGRVVFYDYDEIC
YMTEVNFRDIPQPRYPEDELSGEPWYSVSPGDVFPEEFRHWLCADPRIGPLFEEMHEDLF
RASYWRGLQTRIKNGHVEDVYAYRRKQRFCIRFSPSPCGRGPG