Protein Info for EX28DRAFT_4303 in Enterobacter asburiae PDN3

Annotation: heavy metal-(Cd/Co/Hg/Pb/Zn)-translocating P-type ATPase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 723 transmembrane" amino acids 124 to 140 (17 residues), see Phobius details amino acids 147 to 165 (19 residues), see Phobius details amino acids 181 to 209 (29 residues), see Phobius details amino acids 349 to 369 (21 residues), see Phobius details amino acids 381 to 405 (25 residues), see Phobius details amino acids 676 to 693 (18 residues), see Phobius details amino acids 699 to 720 (22 residues), see Phobius details PF00403: HMA" amino acids 51 to 107 (57 residues), 48.5 bits, see alignment 1.9e-16 TIGR01525: heavy metal translocating P-type ATPase" amino acids 179 to 719 (541 residues), 581.3 bits, see alignment E=3.9e-178 TIGR01512: cadmium-translocating P-type ATPase" amino acids 179 to 721 (543 residues), 535.4 bits, see alignment E=2e-164 TIGR01494: HAD ATPase, P-type, family IC" amino acids 202 to 696 (495 residues), 261.8 bits, see alignment E=1.4e-81 PF00122: E1-E2_ATPase" amino acids 230 to 410 (181 residues), 159.8 bits, see alignment E=1.1e-50 PF00702: Hydrolase" amino acids 427 to 629 (203 residues), 106.1 bits, see alignment E=6.2e-34 PF08282: Hydrolase_3" amino acids 612 to 663 (52 residues), 25.4 bits, see alignment 2.2e-09

Best Hits

Swiss-Prot: 79% identical to ZNTA_SHISS: Zinc/cadmium/lead-transporting P-type ATPase (zntA) from Shigella sonnei (strain Ss046)

KEGG orthology group: K01534, Cd2+/Zn2+-exporting ATPase [EC: 3.6.3.3 3.6.3.5] (inferred from 92% identity to enc:ECL_04831)

MetaCyc: 79% identical to Zn2+/Cd2+/Pb2+ exporting P-type ATPase (Escherichia coli K-12 substr. MG1655)
3.6.3.3-RXN [EC: 7.2.2.21]; RXN0-5205 [EC: 7.2.2.21, 7.2.2.12]; 7.2.2.- [EC: 7.2.2.21, 7.2.2.12]

Predicted SEED Role

"Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)" (EC 3.6.3.3, EC 3.6.3.4, EC 3.6.3.5)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.3, 3.6.3.4, 3.6.3.5

Use Curated BLAST to search for 3.6.3.3 or 3.6.3.4 or 3.6.3.5 or 7.2.2.12 or 7.2.2.21

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (723 amino acids)

>EX28DRAFT_4303 heavy metal-(Cd/Co/Hg/Pb/Zn)-translocating P-type ATPase (Enterobacter asburiae PDN3)
MSTPEIPKKVPQFSALKLSPAPSKDDCCCEGACETPTQPLPESGNRFSWVVNGMDCAACA
RKVENAVKQVPGVSHVQVLFATEKLLVSADNDVSKQVEAAVSKAGYTLRSETAPVEKASS
LKENLPLITLIIMMALSWGLEQINHPFGNLAFIATTLVGLFPIARQALRLMKSGSWFAIE
TLMSVAAIGALFIGATAEAAMVLLLFLIGERLEGWAASRARKGVSALMALKPETATRVIN
GTRETVAINTLRPGDVIEVAAGGRLPADGALLTATASFDESALTGESIPVERAAGEKVPA
GATSVDRLVQLTVLSEPGDSAIDRILKLIEEAEERRAPVERFIDRFSRIYTPAIMLVALL
VTVVPPLFFGAPWEGWIYKGLTLLLIGCPCALVISTPAAITSGLAAAARRGALIKGGAAL
EQLSQVQHIAFDKTGTLTVGKPQVTGVYSQETSEDELLTLAAAVEQGSTHPLAQAIVREA
QSRGLNIPPATAQRALVGSGIEATVDGKKVLIVAAGKSSSPEVEALEQTGQTVVTVLQDG
VAKGMLALRDTLRDDAKEAVAALHKLGVQGVILTGDNPRAAAAIAGELGLEFKAGLLPAD
KVSAVTELNAHAPLAMVGDGINDAPAMKASTIGIAMGSGTDVALETADAALTHNRLTGLA
QMISLARATRANIRQNIGIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLL
RRR