Protein Info for EX28DRAFT_4293 in Enterobacter asburiae PDN3

Annotation: ABC-type branched-chain amino acid transport systems, periplasmic component

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 364 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details PF13433: Peripla_BP_5" amino acids 23 to 311 (289 residues), 58.4 bits, see alignment E=1e-19 PF13458: Peripla_BP_6" amino acids 23 to 341 (319 residues), 179.2 bits, see alignment E=2.8e-56 PF01094: ANF_receptor" amino acids 45 to 354 (310 residues), 82.4 bits, see alignment E=5e-27

Best Hits

Swiss-Prot: 96% identical to LIVJ_CITFR: Leu/Ile/Val-binding protein (livJ) from Citrobacter freundii

KEGG orthology group: K01999, branched-chain amino acid transport system substrate-binding protein (inferred from 99% identity to enc:ECL_04820)

MetaCyc: 94% identical to branched chain amino acid/phenylalanine ABC transporter periplasmic binding protein (Escherichia coli K-12 substr. MG1655)
ABC-15-RXN [EC: 7.4.2.2]; 7.4.2.2 [EC: 7.4.2.2]; 7.4.2.2 [EC: 7.4.2.2]; 7.4.2.2 [EC: 7.4.2.2]

Predicted SEED Role

"High-affinity leucine-specific transport system, periplasmic binding protein LivK (TC 3.A.1.4.1)" in subsystem ABC transporter branched-chain amino acid (TC 3.A.1.4.1) (TC 3.A.1.4.1)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.4.2.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (364 amino acids)

>EX28DRAFT_4293 ABC-type branched-chain amino acid transport systems, periplasmic component (Enterobacter asburiae PDN3)
MKGKALLAGCIALAFSTMAQADIKVAVVGAMSGPVAQYGDQEFTGAEQAVADINAKGGIK
GEKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCSSSTQPASDIYEDEGILMITPA
ATAPELTARGYKLTLRTTGLDSDQGPTAAKYIVEKVKPKRIAIVHDKQQYGEGLARSVQD
NLKKANADVVFFDGITAGEKDFSTLVARLKKENIDFVYYGGYHPEMGQILRQARAAGLKT
QFMGPEGVANVSLSNIAGESAEGLLVTKPKNYDQVPANKPIVDAIKAKKQDPSGAFVWTT
YAALQSLQAGLNQSADPAEIATWLKANTVETVMGPLSWDAKGDLKGFEFGVFDWHANGTA
TDAK