Protein Info for EX28DRAFT_4201 in Enterobacter asburiae PDN3

Annotation: phosphonate ABC transporter, periplasmic phosphonate binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 338 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details TIGR03431: phosphonate ABC transporter, periplasmic phosphonate binding protein" amino acids 4 to 291 (288 residues), 364.1 bits, see alignment E=5.5e-113 TIGR01098: phosphate/phosphite/phosphonate ABC transporter, periplasmic binding protein" amino acids 5 to 254 (250 residues), 304.5 bits, see alignment E=6e-95 PF12974: Phosphonate-bd" amino acids 35 to 277 (243 residues), 261.7 bits, see alignment E=2.9e-82

Best Hits

Swiss-Prot: 88% identical to PHND_ECOLI: Phosphonates-binding periplasmic protein (phnD) from Escherichia coli (strain K12)

KEGG orthology group: K02044, phosphonate transport system substrate-binding protein (inferred from 98% identity to enc:ECL_00361)

Predicted SEED Role

"Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1)" in subsystem ABC transporter alkylphosphonate (TC 3.A.1.9.1) (TC 3.A.1.9.1)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (338 amino acids)

>EX28DRAFT_4201 phosphonate ABC transporter, periplasmic phosphonate binding protein (Enterobacter asburiae PDN3)
MSYKAVAALAFTSMFSLSTLLSPAYAQEQEKALNFGIISTESQQNLKPQWEPFLKDMETK
LGIKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRANGQVFAQTVAADGSPGYW
SVLIVNKDSPINNLNDMLAKRKELTFGNGDPNSTSGYLVPGYYVFAKNNASASDFKRTVN
ASHETNALAVANKQVDVATNNTENLDKLKTSAPDKLKEIKVIWKSPLIPGDPIVWRKNLS
ESTKDKVYDFFMTYGKTPEEKAVLARLGWAPFRPSSDLQLVPIRQLALFKEMQGVKDNKG
LKDEEKTSKVATIQAQLEDLDRLTAALGAMTSENKAVQ