Protein Info for EX28DRAFT_4162 in Enterobacter asburiae PDN3

Annotation: nitrite reductase [NAD(P)H], large subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 847 PF07992: Pyr_redox_2" amino acids 5 to 305 (301 residues), 189.6 bits, see alignment E=3e-59 TIGR02374: nitrite reductase [NAD(P)H], large subunit" amino acids 6 to 801 (796 residues), 1344.9 bits, see alignment E=0 PF13738: Pyr_redox_3" amino acids 83 to 280 (198 residues), 35.4 bits, see alignment E=2.5e-12 PF00070: Pyr_redox" amino acids 148 to 227 (80 residues), 64.8 bits, see alignment E=3e-21 PF18267: Rubredoxin_C" amino acids 321 to 388 (68 residues), 39.2 bits, see alignment E=1.9e-13 PF04324: Fer2_BFD" amino acids 424 to 471 (48 residues), 54.2 bits, see alignment 5.4e-18 PF03460: NIR_SIR_ferr" amino acids 560 to 622 (63 residues), 42 bits, see alignment 2.3e-14 PF01077: NIR_SIR" amino acids 633 to 757 (125 residues), 69.8 bits, see alignment E=6.4e-23

Best Hits

Swiss-Prot: 93% identical to NIRB_ECOLI: Nitrite reductase (NADH) large subunit (nirB) from Escherichia coli (strain K12)

KEGG orthology group: K00362, nitrite reductase (NAD(P)H) large subunit [EC: 1.7.1.4] (inferred from 98% identity to enc:ECL_04742)

MetaCyc: 93% identical to nitrite reductase (NADH) large subunit (Escherichia coli K-12 substr. MG1655)
RXN-13854 [EC: 1.7.1.15]

Predicted SEED Role

"Nitrite reductase [NAD(P)H] large subunit (EC 1.7.1.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.7.1.4

Use Curated BLAST to search for 1.7.1.15 or 1.7.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (847 amino acids)

>EX28DRAFT_4162 nitrite reductase [NAD(P)H], large subunit (Enterobacter asburiae PDN3)
MSKVRLAIIGNGMVGHRFIEDLLDKADAEQFDITVFCEEPRKAYDRVHLSSYFSHHTAEE
LSLVREGFYEKHGVKVLVGERAITINRQEKVIHSSAGRTVFYDKLIMATGSYPWIPPIKG
SETQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEAAGALKNLGVETHVIEFAPML
MAEQLDHMGGDQLRRKIESMGVKVHTSKNTKEIVQEGTEARKTMRFADGSELEVDFIVFS
TGIRPRDKLATQCGLAVAQRGGIMINDACQTSDPDIYAIGECASWNNRVYGLVAPGYKMA
QVAVDHILGSENAFTGADMSAKLKLLGVDVGGIGDAHGRTPNSRSYVYLDESKEVYKRLI
VSQDNKTLLGAVLVGDTSDFGNLLQLVLNAIELPENPDALILPAHASSGKPSIGVDKLPD
SAQICSCFDVTKGMLISAINKGCHTVAALKAETKAGTGCGGCIPLVTQVLNAELAKQGIE
VNNNLCEHFTYSRQELYHLIRVEGIKSFDELLAKHGQGYGCEVCKPTVGSLLASCWNEYV
LKPEHTPLQDTNDNFLANIQKDGTYSVIPRSAGGEITPEGLVAVGRIAREFNLYTKITGS
QRIGLFGAQKDDLPEIWRQLIEAGFETGHAYAKALRMAKTCVGSTWCRYGVGDSVGFGVE
LENRYKGIRTPHKMKFGVSGCTRECAEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLL
AADLDRDTLIQYLDRFMMFYIRTADKLTRTASWLDNLEGGIDYLKSVIIDDKLGLNEHLE
AEMARLREAVICEWTETVNTPAAQTRFKHFINSTQRDPNVQVVPEREQHRPATPYERIPV
TLVEENA