Protein Info for EX28DRAFT_4130 in Enterobacter asburiae PDN3
Annotation: pimelyl-[acyl-carrier protein] methyl ester esterase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 78% identical to BIOH_CITK8: Pimeloyl-[acyl-carrier protein] methyl ester esterase (bioH) from Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696)
KEGG orthology group: K02170, biotin biosynthesis protein BioH (inferred from 84% identity to ent:Ent638_3825)MetaCyc: 79% identical to pimeloyl-acyl carrier protein methyl ester esterase (Escherichia coli K-12 substr. MG1655)
RXN-11483 [EC: 3.1.1.85]; Carboxylesterase. [EC: 3.1.1.85, 3.1.1.1]
Predicted SEED Role
"Biotin synthesis protein BioH"
MetaCyc Pathways
- biotin biosynthesis I (15/15 steps found)
- 8-amino-7-oxononanoate biosynthesis I (11/11 steps found)
- methylsalicylate degradation (2/2 steps found)
- aspartame degradation (1/2 steps found)
- methyl indole-3-acetate interconversion (1/2 steps found)
- superpathway of methylsalicylate metabolism (1/5 steps found)
- retinol biosynthesis (2/7 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 3.1.1.1
Use Curated BLAST to search for 3.1.1.1 or 3.1.1.85
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (257 amino acids)
>EX28DRAFT_4130 pimelyl-[acyl-carrier protein] methyl ester esterase (Enterobacter asburiae PDN3) MKTLWWQTVGTGNCHLVLLHGWGLNAEIWHCIREELASHFTLHLVDLPGFGRSRGYGALS LDEMVQQVLDAAPQNAVWLGWSLGGLVASQIALSRPDRVKALVTVASSPCFSAQEEWPGI KPDVLAGFQQQLSDDFQRTVERFLALQTMGTDTARKDARTLKQTVLSLPMPEVEVLNRGL ELLKTVDLREPLAALTMPHLRIYGYLDGIVPRKVVPLLDSLWPDSESQIIAKAAHAPFIS HPTEFCSALIALSQRFD