Protein Info for EX28DRAFT_4126 in Enterobacter asburiae PDN3

Annotation: 4-alpha-glucanotransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 692 PF21226: MalQ_N" amino acids 52 to 127 (76 residues), 59.3 bits, see alignment E=3.2e-20 TIGR00217: 4-alpha-glucanotransferase" amino acids 99 to 684 (586 residues), 936.1 bits, see alignment E=4.8e-286 PF02446: Glyco_hydro_77" amino acids 147 to 663 (517 residues), 443.3 bits, see alignment E=1.1e-136

Best Hits

Swiss-Prot: 87% identical to MALQ_ECOLI: 4-alpha-glucanotransferase (malQ) from Escherichia coli (strain K12)

KEGG orthology group: K00705, 4-alpha-glucanotransferase [EC: 2.4.1.25] (inferred from 96% identity to enc:ECL_04780)

MetaCyc: 87% identical to 4-alpha-glucanotransferase (Escherichia coli K-12 substr. MG1655)
4-alpha-glucanotransferase. [EC: 2.4.1.25]; 2.4.1.25 [EC: 2.4.1.25]; 2.4.1.25 [EC: 2.4.1.25]; MALTODEG-RXN [EC: 2.4.1.25]; RXN0-7347 [EC: 2.4.1.25]

Predicted SEED Role

"4-alpha-glucanotransferase (amylomaltase) (EC 2.4.1.25)" in subsystem Glycogen metabolism or Maltose and Maltodextrin Utilization (EC 2.4.1.25)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.1.25

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (692 amino acids)

>EX28DRAFT_4126 4-alpha-glucanotransferase (Enterobacter asburiae PDN3)
MESKRLDSAAQAAGISLSYINAHGKPQSIGADTKRRLLDAMHKADAKASVAPVPNVKVFT
AGKKMPLAVEGRGEFSWLLTTEEGHQHKGHATGGKTLNLPAKLPEGYHTLTLTQDDLRFH
CRVIVAPKRCYEPQALLEGKKLWGACVQLYTLRSDSNWGIGDFGDLKKMLVDVGERGGAF
IGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNALDDFKNSKEAQAWWKLSTTQQAL
KQARDADWVDYSTVTALKMAALRLAWKGFSQRDDEQMAAFRQFVTQEGESLYWQAAFDAL
HAYQVKEDEMRWGWPVWPDAYQSVDTPEVKAFCEKYADEMDFYLWLQWLAYSQFAACWQV
SQGYNMPIGLYRDLAVGVAEGGAETWCDRELYCLKASVGAPPDILGPLGQNWGLPPMDPH
VMAARAYEPFIDLLRANMQNCGALRIDHVMSVLRLWWIPYGETADHGAYVQYPVDDLLSI
LALESKRHQCMVIGEDLGTVPVEIVSKLRDSGVYSYKVLYFENDHEKTFRAPKAYPEQSM
AVATTHDLPTLRGYWESGDLTLGKTLGLYPDEEVLRGLYQDRELSKQGLLDALHKQGCLP
KRAGHKASLMSMTATLNRGLQRYIADSNSALLGLQPEDWIDMAEPVNIPGTSYQYKNWRR
KLSTTLEKMFADDGVNKLIKDLDKRRKAAAKK