Protein Info for EX28DRAFT_4115 in Enterobacter asburiae PDN3

Annotation: alpha-1,4-glucan:alpha-1,4-glucan 6-glycosyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 758 TIGR01515: 1,4-alpha-glucan branching enzyme" amino acids 139 to 753 (615 residues), 1080.7 bits, see alignment E=0 PF02922: CBM_48" amino acids 155 to 237 (83 residues), 95.9 bits, see alignment E=2.2e-31 PF00128: Alpha-amylase" amino acids 303 to 375 (73 residues), 32.3 bits, see alignment E=1.2e-11 PF02806: Alpha-amylase_C" amino acids 658 to 754 (97 residues), 88.6 bits, see alignment E=4.2e-29

Best Hits

Swiss-Prot: 93% identical to GLGB_ENT38: 1,4-alpha-glucan branching enzyme GlgB (glgB) from Enterobacter sp. (strain 638)

KEGG orthology group: K00700, 1,4-alpha-glucan branching enzyme [EC: 2.4.1.18] (inferred from 97% identity to enc:ECL_04794)

MetaCyc: 92% identical to 1,4-alpha-glucan branching enzyme (Escherichia coli K-12 substr. MG1655)
1,4-alpha-glucan branching enzyme. [EC: 2.4.1.18]

Predicted SEED Role

"1,4-alpha-glucan (glycogen) branching enzyme, GH-13-type (EC 2.4.1.18)" in subsystem Glycogen metabolism or Trehalose Biosynthesis (EC 2.4.1.18)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.1.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (758 amino acids)

>EX28DRAFT_4115 alpha-1,4-glucan:alpha-1,4-glucan 6-glycosyltransferase (Enterobacter asburiae PDN3)
MHIFVLSRSGHIKSRPGALKKDDIKNRMNTMSDRVDRDVINALIAGHFADPFSVLGMHQT
EDGLEVRALLPDATEVWVIEPKTGRKVGNLECLDSRGFFSGVMPRRKNPFRYQLAVLWHG
QQNLIDDPYSFGPLLKEMDAWLLSEGTHLRPYETLGAHADTMDGITGTRFSVWAPNAQRV
SVVGQFNYWDGRRHPMRLRRETGIWELFIPGAHPGQLYKFEMIDANGKLRIKADPYAFEA
QMRPDTASLICGLPEKVVQTEERKQANRFDAPISVYEVHLGSWRRHTDNNFWLSYRELAD
QLVPYAKWMGFTHLELLPVNEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGL
NVILDWVPGHFPSDDFALAEFDGTKLYEHSDPREGYHQDWNTLIYNYGRREVANYLVGNA
LYWIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRIIGEQV
DGAVTMAEESTDFAGVSRPPSLGGLGFWYKWNLGWMHDTLDYMKLDPVHRKYHHDKLTFG
MLYNYTENFMLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMFAFPGKKLLFMGN
EFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKALHELDFDPYGFEWLV
VDDHERSVFVFVRRDKAGNEIIVASNFTPVPREHYRFGINQPGKWREILNTDSMHYHGSN
AGNGGLVQSDAIESHGRPNSLSLTLPPLGTIWLMREGE