Protein Info for EX28DRAFT_4084 in Enterobacter asburiae PDN3

Annotation: Methionyl-tRNA formyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 660 transmembrane" amino acids 295 to 311 (17 residues), see Phobius details PF00551: Formyl_trans_N" amino acids 26 to 176 (151 residues), 92.4 bits, see alignment E=6.3e-30 PF02911: Formyl_trans_C" amino acids 203 to 295 (93 residues), 70.8 bits, see alignment E=1.8e-23 PF01370: Epimerase" amino acids 318 to 566 (249 residues), 153 bits, see alignment E=2e-48 PF16363: GDP_Man_Dehyd" amino acids 319 to 644 (326 residues), 89 bits, see alignment E=8.8e-29

Best Hits

Swiss-Prot: 85% identical to ARNA_KLEP7: Bifunctional polymyxin resistance protein ArnA (arnA) from Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)

KEGG orthology group: K10011, UDP-4-amino-4-deoxy-L-arabinose formyltransferase / UDP-glucuronic acid dehydrogenase (UDP-4-keto-hexauronic acid decarboxylating) [EC: 1.1.1.305 2.1.2.13] (inferred from 95% identity to enc:ECL_04861)

MetaCyc: 77% identical to fused UDP-4-amino-4-deoxy-L-arabinose formyltransferase/UDP-glucuronate dehydrogenase (Escherichia coli K-12 substr. MG1655)
RXN0-1862 [EC: 2.1.2.13]; RXN0-1861 [EC: 2.1.2.13, 1.1.1.305]

Predicted SEED Role

"UDP-glucuronic acid oxidase (UDP-4-keto-hexauronic acid decarboxylating) (EC 1.1.1.305) / UDP-4-amino-4-deoxy-L-arabinose formyltransferase (EC 2.1.2.13)" (EC 1.1.1.305, EC 2.1.2.13)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.305 or 2.1.2.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (660 amino acids)

>EX28DRAFT_4084 Methionyl-tRNA formyltransferase (Enterobacter asburiae PDN3)
MKAVVFAYHDMGCTGTLALLEAGYDIAAIFTHPDTAGENNFFGSVARIAAERGIPVYAPD
DVNHPLWVDRIRALAPDVIFSFYYRNLLSDEILGLAAKGAFNLHGSLLPAYRGRAPLNWV
LVNGETETGVTLHRMVRRADAGAIVAQQKVAIDADETALQLHQKLNATAQTLLRDALPAI
LSGTFSETAQDESKASSFGRRAPEDGRLDWNKPARQLHNLVRAVTDPWPGAYSFAGVSKF
IVWKSRVRDDIPAAKPGTVVSVTPLIVSCGEQALEIVTGQTDNGLYVQGTQLAQSLGLVA
GALITSAPVVAIKRRTRVLILGVNGFIGNHLTERLLKDDNYEIYGLDIGSDAISRFLDNP
RFHFVEGDISIHSEWIEYHIKKCDVVLPLVAIATPIEYTRNPLRVFELDFEENLKIIRDC
VKYDKRIIFPSTSEVYGMCTDKNFDEDTSNLVVGPINKQRWIYSVSKQLLDRVIWAYGEK
EGLRFTLFRPFNWMGPRLDNLNAARIGSSRAITQLILNLVEGSPIKLIEGGKQKRCFTDI
SDGIEALFRIIENKDNRCNGEIINIGNPDNEASIRELAEMLLASFERHPLRDKFPPFAGF
REVESSSYYGKGYQDVEHRKPSIRNAKRCLNWTPTVKMELTIDETLDFFLRTVELSEQDS