Protein Info for EX28DRAFT_3993 in Enterobacter asburiae PDN3

Annotation: tRNA modification GTPase TrmE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 454 PF10396: TrmE_N" amino acids 6 to 120 (115 residues), 124.9 bits, see alignment E=4.1e-40 TIGR00450: tRNA modification GTPase TrmE" amino acids 11 to 454 (444 residues), 329.9 bits, see alignment E=2.9e-102 PF12631: MnmE_helical" amino acids 123 to 451 (329 residues), 212.2 bits, see alignment E=2e-66 TIGR00231: small GTP-binding protein domain" amino acids 216 to 371 (156 residues), 79.6 bits, see alignment E=2.3e-26 PF01926: MMR_HSR1" amino acids 218 to 336 (119 residues), 90 bits, see alignment E=2.3e-29 PF02421: FeoB_N" amino acids 218 to 306 (89 residues), 30.7 bits, see alignment E=4.4e-11

Best Hits

Swiss-Prot: 96% identical to MNME_ENT38: tRNA modification GTPase MnmE (mnmE) from Enterobacter sp. (strain 638)

KEGG orthology group: K03650, tRNA modification GTPase (inferred from 99% identity to enc:ECL_05163)

MetaCyc: 95% identical to 5-carboxymethylaminomethyluridine-tRNA synthase GTPase subunit (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"GTPase and tRNA-U34 5-formylation enzyme TrmE" in subsystem Universal GTPases

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (454 amino acids)

>EX28DRAFT_3993 tRNA modification GTPase TrmE (Enterobacter asburiae PDN3)
MSHNDTIVAQATPPGRGGVGILRISGLKAREVAEAVLGKLPKPRYADYLPFKDTDGTPLD
QGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTLPGLRIAKPGEFSERAFLNDK
LDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLVEALTHLRIYVEAAIDFPDEE
IDFLSDGKIEAQLNQVMNDLDAVRAEARQGSLLREGMKVVIAGRPNAGKSSLLNALAGRE
AAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRDASDEVERIGIERAWQEIEQADRVL
FMVDGTTTDAVDPAEIWPDFIARLPAKLPITVVRNKADVTGETLGISDVNGHSLIRLSAR
TGEGVDDLRNHLKQSMGFDTSMEGGFLARRRHLQALEEAANHLVQGKAQLIGAWAGELLA
EELRLAQQNLSEITGEFTSDDLLGRIFSSFCIGK