Protein Info for EX28DRAFT_3987 in Enterobacter asburiae PDN3

Annotation: DNA replication and repair protein RecF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 357 PF13175: AAA_15" amino acids 1 to 46 (46 residues), 38.2 bits, see alignment 3.5e-13 TIGR00611: DNA replication and repair protein RecF" amino acids 1 to 355 (355 residues), 454.7 bits, see alignment E=1.2e-140 PF13514: AAA_27" amino acids 1 to 111 (111 residues), 25.8 bits, see alignment E=1.9e-09 PF02463: SMC_N" amino acids 3 to 355 (353 residues), 160.9 bits, see alignment E=8.1e-51 PF13476: AAA_23" amino acids 5 to 45 (41 residues), 34.6 bits, see alignment 7.9e-12 PF13304: AAA_21" amino acids 25 to 47 (23 residues), 27.3 bits, see alignment (E = 8.8e-10)

Best Hits

Swiss-Prot: 97% identical to RECF_ENT38: DNA replication and repair protein RecF (recF) from Enterobacter sp. (strain 638)

KEGG orthology group: K03629, DNA replication and repair protein RecF (inferred from 99% identity to enc:ECL_00003)

MetaCyc: 96% identical to recombination mediator protein RecF (Escherichia coli K-12 substr. MG1655)
RXN0-2606

Predicted SEED Role

"DNA recombination and repair protein RecF" in subsystem DNA repair, bacterial RecFOR pathway

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (357 amino acids)

>EX28DRAFT_3987 DNA replication and repair protein RecF (Enterobacter asburiae PDN3)
MSLTRLLIRDFRNIESADLALSPGFNFLVGANGSGKTSVLEAIYTLGHGRAFRSLQIGRV
IRHEQESFVLHGRLQGAERETAIGLTKDKQGDSKVRIDGTDGHKVAELALLMPMQLITPE
GFTLLNGGPKYRRAFLDWGCFHNEAGFFNAWSNLKRLLKQRNAALRQVTRYAQLRPWDME
LIPLAEQISNWRAEYSAGIAEDMADTCKQFLPEFSLTFSFQRGWEKETDYAEVLERSFER
DRMLTYTAHGPHKADFRIRADGAPVEDTLSRGQLKLLMCALRLAQGEFLTRESGRRCLYL
IDDFASELDDARRGLLASRLKATQSQVFVSAISAEHVIDMSDENSKMFTVEKGKITD