Protein Info for EX28DRAFT_3986 in Enterobacter asburiae PDN3

Annotation: DNA gyrase, B subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 803 TIGR01059: DNA gyrase, B subunit" amino acids 5 to 803 (799 residues), 1037.3 bits, see alignment E=0 PF02518: HATPase_c" amino acids 32 to 171 (140 residues), 63.6 bits, see alignment E=7e-21 PF00204: DNA_gyraseB" amino acids 221 to 390 (170 residues), 203.3 bits, see alignment E=6.1e-64 PF01751: Toprim" amino acids 419 to 531 (113 residues), 67.6 bits, see alignment E=2.7e-22 PF21249: GyrB_hook" amino acids 533 to 561 (29 residues), 66.7 bits, see alignment (E = 3.2e-22) PF18053: GyrB_insert" amino acids 564 to 728 (165 residues), 171.6 bits, see alignment E=4.3e-54 PF00986: DNA_gyraseB_C" amino acids 732 to 792 (61 residues), 106.5 bits, see alignment 1.8e-34

Best Hits

Swiss-Prot: 96% identical to GYRB_SALTI: DNA gyrase subunit B (gyrB) from Salmonella typhi

KEGG orthology group: K02470, DNA gyrase subunit B [EC: 5.99.1.3] (inferred from 96% identity to esc:Entcl_0004)

MetaCyc: 94% identical to DNA gyrase subunit B (Escherichia coli K-12 substr. MG1655)
5.99.1.3-RXN [EC: 5.6.2.2]

Predicted SEED Role

"DNA gyrase subunit B (EC 5.99.1.3)" in subsystem DNA topoisomerases, Type II, ATP-dependent or Resistance to fluoroquinolones (EC 5.99.1.3)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.3

Use Curated BLAST to search for 5.6.2.2 or 5.99.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (803 amino acids)

>EX28DRAFT_3986 DNA gyrase, B subunit (Enterobacter asburiae PDN3)
MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNAIDEALAGHCKDIV
VTIHADNSVSVTDDGRGIPTGIHPEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVGV
SVVNALSQKLELVIQREGKIHRQLYTHGVPQAPLAVTGDTDKTGTMVRFWPSHETFTNVT
EFEYDILAKRLRELSFLNSGVSIRLKDKRDGKEDHFHYEGGIKAFVEYLNKNKTPIHPNI
FYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQRDGGTHLAGFRAAMTRTLNAYMDKE
GYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSSQTKDKLVSSEVKSAVEQQMNELLSEY
LLENPSDAKIVVGKIIDAARAREAARKAREMTRRKGALDLAGLPGKLADCQERDPALSEL
YLVEGDSAGGSAKQGRNRKNQAILPLKGKILNVEKARFDKMLSSQEVATLITALGCGIGR
DEYNPDKLRYHSIIIMTDADVDGSHIRTLLLTFFYRQMPEIVERGHVYIAQPPLYKVKKG
KQEQYIKDDEAMDQYQIAIALDGATLHANSHAPALAGEPLERLVSEFNATQKMITRMERR
YPKTLLKELVYQPTLTEADLSDEQTVTRWVNALVTDLNEKEQHGSMWKCDIQQNADKQFE
PIIRVRTHGVDTDYPLEHEFVTGPEYRRICTLGETLRGLIEDDAFIERGERRQPVASFEQ
ALDWLVKESRRGLAIQRYKGLGEMNPDQLWETTMDPESRRMLRVTVKDAIAADQLFTTLM
GDAVEPRRAFIEENALKAANIDI