Protein Info for EX28DRAFT_3979 in Enterobacter asburiae PDN3

Annotation: L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02746: MR_MLE_N
  • amino acids 15 to 107 (93 residues), 105.9 bits, E=1.6e-34
• PF13378: MR_MLE_C
  • amino acids 134 to 355 (222 residues), 212.1 bits, E=8.6e-67

Best Hits

Swiss-Prot: 97% identical to DGOD_ENT38: D-galactonate dehydratase (dgoD) from Enterobacter sp. (strain 638)

KEGG orthology group: K01684, galactonate dehydratase [EC: 4.2.1.6] (inferred from 99% identity to enc:ECL_00011)

MetaCyc: 94% identical to D-galactonate dehydratase (Escherichia coli K-12 substr. MG1655)
Galactonate dehydratase. [EC: 4.2.1.140, 4.2.1.6]

Predicted SEED Role

"Galactonate dehydratase (EC 4.2.1.6)" in subsystem D-galactonate catabolism (EC 4.2.1.6)

MetaCyc Pathways

• Entner-Doudoroff pathway II (non-phosphorylative) (8/9 steps found)
• D-galactonate degradation (3/3 steps found)
• Entner-Doudoroff pathway III (semi-phosphorylative) (7/9 steps found)

KEGG Metabolic Maps

• Galactose metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.1.140 or 4.2.1.6

Protein Sequence (382 amino acids)

>EX28DRAFT_3979 L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily (Enterobacter asburiae PDN3)
MKITKLTTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAAVHELGEYLIGQDPARI
NDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPVWQLMGGLVRDKIKAYSWV
GGDRPAEVIDGITQLRNIGFDTFKLNGCEEMGIIDNSRAVDRAVNTVAQIREAFGNEIEF
GLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAEYYPKLAEQTHIPIAAGERMFSR
FEFKRVLEAGGIAILQPDLSHAGGITECYKIAGMAEAYDVALAPHCPLGPIALAACLHVD
FVSRNAVFQEQSMGIHYNKGAELLDFVKNKEDFCMEGGFFKPLTKPGLGVEIDEAKVIEL
SKNAPDWRNPLWRHEDGSVAEW