Protein Info for EX28DRAFT_3896 in Enterobacter asburiae PDN3

Annotation: NAD-dependent DNA ligase (contains BRCT domain type II)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 556 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details PF01653: DNA_ligase_aden" amino acids 27 to 305 (279 residues), 100.8 bits, see alignment E=8.6e-33 PF03120: DNA_ligase_OB" amino acids 310 to 383 (74 residues), 77.5 bits, see alignment E=5.9e-26

Best Hits

Swiss-Prot: 61% identical to LIGB_ENT38: DNA ligase B (ligB) from Enterobacter sp. (strain 638)

KEGG orthology group: K01972, DNA ligase (NAD+) [EC: 6.5.1.2] (inferred from 81% identity to enc:ECL_00115)

MetaCyc: 58% identical to DNA ligase B (Escherichia coli K-12 substr. MG1655)
DNA ligase (NAD(+)). [EC: 6.5.1.2, 6.5.1.6]

Predicted SEED Role

"DNA ligase (EC 6.5.1.2), LigB" (EC 6.5.1.2)

Isozymes

Compare fitness of predicted isozymes for: 6.5.1.2

Use Curated BLAST to search for 6.5.1.2 or 6.5.1.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (556 amino acids)

>EX28DRAFT_3896 NAD-dependent DNA ligase (contains BRCT domain type II) (Enterobacter asburiae PDN3)
MWRWISGLMLLWCGYGAAVCPVWSQAKAEKEMASLSAQIKRWDEAYWKQGVSEINDEVYD
QLHGRLTQWQRCFGSETSEDKLPTLTGSVKHPVAHTGVHKVASKEELRQWMRVRQHLWLQ
PKVDGVAVTLVYRNGKLTQAISRGDGLTGEDWTGKVRLIPSVPQKLTGALANSVLQGELF
WLRDNHIQNQMGGMNARAKVAGAMMRQKDNALLSKIGVFIWAWPDGPKDMRTSLAELSQA
GFPLSARYTLPADSVDAVEKQRAAWHVTALPFATDGIVVRSEDEPAGEEWLPGEGSWVVA
WKYPPSAQVAEVRDIHFSVGRTGKISVVAALEPVQLDDKQVQRVSLGSVGRWQNMDIAPG
DQIQISLAGQGIPRFDKVLWRGTDRHKPEPPAPRYHALSCFYASPECMEQFFARLTWLSS
KQVFDIEGLGESGWRTLWQAHRFEHLFSWLLLTQEQLQATPGFSSARGLALWHRFNLVRD
KPFIRWLMALSVPMTQASLKAMGDISWQEMSGRNAKDWQALPGTGEEKARQIVNWIHAPQ
VDVLAKWLAEQHINGF