Protein Info for EX28DRAFT_3889 in Enterobacter asburiae PDN3

Annotation: phosphopantothenoylcysteine decarboxylase / phosphopantothenate--cysteine ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00521: phosphopantothenoylcysteine decarboxylase / phosphopantothenate--cysteine ligase
  • amino acids 4 to 395 (392 residues), 541.7 bits, E=5.2e-167
• PF02441: Flavoprotein
  • amino acids 7 to 176 (170 residues), 109.8 bits, E=1.1e-35
• PF04127: DFP
  • amino acids 187 to 396 (210 residues), 283.6 bits, E=1.1e-88

Best Hits

Swiss-Prot: 92% identical to COABC_ECOLI: Coenzyme A biosynthesis bifunctional protein CoaBC (coaBC) from Escherichia coli (strain K12)

KEGG orthology group: K13038, phosphopantothenoylcysteine decarboxylase / phosphopantothenate--cysteine ligase [EC: 4.1.1.36 6.3.2.5] (inferred from 97% identity to enc:ECL_00122)

MetaCyc: 92% identical to fused 4'-phosphopantothenoylcysteine decarboxylase and phosphopantothenoylcysteine synthetase (Escherichia coli K-12 substr. MG1655)
Phosphopantothenate--cysteine ligase. [EC: 6.3.2.5]; Phosphopantothenoylcysteine decarboxylase. [EC: 6.3.2.5, 4.1.1.36]

Predicted SEED Role

"Phosphopantothenoylcysteine decarboxylase (EC 4.1.1.36) / Phosphopantothenoylcysteine synthetase (EC 6.3.2.5)" in subsystem Coenzyme A Biosynthesis (EC 4.1.1.36, EC 6.3.2.5)

MetaCyc Pathways

• superpathway of coenzyme A biosynthesis I (bacteria) (9/9 steps found)
• superpathway of coenzyme A biosynthesis III (mammals) (5/5 steps found)
• coenzyme A biosynthesis I (bacteria) (4/4 steps found)
• coenzyme A biosynthesis II (eukaryotic) (4/4 steps found)
• coenzyme A biosynthesis III (archaea) (3/4 steps found)
• taurine biosynthesis II (1/4 steps found)

KEGG Metabolic Maps

• Pantothenate and CoA biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.1.36 or 6.3.2.5

Protein Sequence (404 amino acids)

>EX28DRAFT_3889 phosphopantothenoylcysteine decarboxylase / phosphopantothenate--cysteine ligase (Enterobacter asburiae PDN3)
MMSLAGKKIVLGVSGGIAAYKAPELVRRLRERGADVRVAITEGGKAFITPLSLQAVSGYP
VSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGMANDLVSTICLATPAPVA
VVPAMNQQMYRNAATQHNLETLASRGLLIWGPDSGSQACGDVGPGRMLDPLTIVDMAAAH
FSPVNDLQHLNIMITAGPTREPLDPVRYITNHSSGKMGFAIAAAAARRGANVTLVSGPVS
LPTPAFVKRIDVTTALEMEAAVQAHAQSQQIFIGCAAVADYRAETIAEAKIKKQGDELTL
KMVKNPDIVAGVAALKSHRPYVVGFAAETNNVEEYARQKRTRKNLDLICANDVSLATQGF
NSDSNALHLFWQDGDKVLPLERKELLGQQLLDEIVTRYDEKNRR