Protein Info for EX28DRAFT_3849 in Enterobacter asburiae PDN3

Annotation: PTS system, mannitol-specific IIC component

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 635 transmembrane" amino acids 20 to 42 (23 residues), see Phobius details amino acids 51 to 72 (22 residues), see Phobius details amino acids 79 to 107 (29 residues), see Phobius details amino acids 130 to 153 (24 residues), see Phobius details amino acids 160 to 181 (22 residues), see Phobius details amino acids 213 to 234 (22 residues), see Phobius details amino acids 244 to 263 (20 residues), see Phobius details amino acids 269 to 292 (24 residues), see Phobius details amino acids 312 to 334 (23 residues), see Phobius details TIGR00851: PTS system, mannitol-specific IIC component" amino acids 8 to 347 (340 residues), 617.3 bits, see alignment E=3.2e-190 PF02378: PTS_EIIC" amino acids 17 to 277 (261 residues), 84.1 bits, see alignment E=1.5e-27 PF02302: PTS_IIB" amino acids 379 to 454 (76 residues), 41.5 bits, see alignment E=2.6e-14 PF00359: PTS_EIIA_2" amino acids 498 to 635 (138 residues), 117.4 bits, see alignment E=7.9e-38

Best Hits

Swiss-Prot: 93% identical to PTM3C_ECOLI: PTS system mannitol-specific EIICBA component (mtlA) from Escherichia coli (strain K12)

KEGG orthology group: K02798, PTS system, mannitol-specific IIA component [EC: 2.7.1.69] K02799, PTS system, mannitol-specific IIB component [EC: 2.7.1.69] K02800, PTS system, mannitol-specific IIC component (inferred from 98% identity to enc:ECL_00170)

MetaCyc: 93% identical to mannitol-specific PTS enzyme IICBA component (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-169 [EC: 2.7.1.198]; TRANS-RXN-156 [EC: 2.7.1.198, 2.7.1.197]

Predicted SEED Role

"PTS system, mannitol-specific IIC component (EC 2.7.1.69) / PTS system, mannitol-specific IIB component (EC 2.7.1.69) / PTS system, mannitol-specific IIA component" in subsystem Mannitol Utilization (EC 2.7.1.69)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.69

Use Curated BLAST to search for 2.7.1.197 or 2.7.1.198 or 2.7.1.69

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (635 amino acids)

>EX28DRAFT_3849 PTS system, mannitol-specific IIC component (Enterobacter asburiae PDN3)
MSSDFKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLPNETLAKLVGPMITYLL
PLLIGFTGGRLVGGDRGGVVGAITTMGVIVGADMPMFLGAMIAGPLGGWAIKKFDVWVDG
KIKSGFEMLVNNFSAGIIGMILAILAFLGIGPAVEVLSKILAAGVNFMVAHDMLPLASIF
VEPAKILFLNNAINHGIFSPLGIQQSHDLGKSIFFLIEANPGPGMGVLLAYMFFGRGSAK
QSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILGGMTGVFTLSVLGGGLVSPASPGSI
LAVLAMTPKGAYFANIAAICAAMAVSFVVSSILLKTSKVKEDDDIEAATRRMHDMKAESK
GATPLAAGDVSNDLSHVRKIIVACDAGMGSSAMGAGVLRKKVQDAGLTNISVTNSAINSL
PPDVDLVITHRDLTERAMRQVPQAQHISLTNFLDSGLYTSLTERLVAAQRHEDNEVKVRS
SLQDSFDESNAHLFKLGAENIFLGRTAATKEEAIRFAGEQLVKGGYVQPEYVDAMLEREK
LTPTYLGESIAVPHGTVEAKDRVLKTGVVFCQYPQGVRFGEEEDDIARLVIGIAARNNEH
IQVITSLTNALDDESVIARLASTTSVEEVLALLNK