Protein Info for EX28DRAFT_3844 in Enterobacter asburiae PDN3

Annotation: selenocysteine-specific elongation factor SelB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00475: selenocysteine-specific translation elongation factor
  • amino acids 1 to 607 (607 residues), 849.8 bits, E=5.8e-260
• PF00009: GTP_EFTU
  • amino acids 2 to 163 (162 residues), 121 bits, E=1.7e-38
• PF01926: MMR_HSR1
  • amino acids 3 to 108 (106 residues), 24.9 bits, E=6.7e-09
• PF03144: GTP_EFTU_D2
  • amino acids 189 to 256 (68 residues), 38.9 bits, E=3.4e-13
• PF21458: SelB-wing_1_3
  • amino acids 350 to 397 (48 residues), 81.6 bits, 9.8e-27
• PF09106: SelB-wing_2
  • amino acids 424 to 480 (57 residues), 58.6 bits, 1.8e-19
• PF21214: bact_SelB_WH_2nd
  • amino acids 487 to 545 (59 residues), 108.1 bits, 5.4e-35
• PF09107: SelB-wing_3
  • amino acids 561 to 605 (45 residues), 55.6 bits, 1e-18

Best Hits

Swiss-Prot: 86% identical to SELB_ECOLI: Selenocysteine-specific elongation factor (selB) from Escherichia coli (strain K12)

KEGG orthology group: K03833, selenocysteine-specific elongation factor (inferred from 92% identity to enc:ECL_00176)

MetaCyc: 86% identical to selenocysteyl-tRNA-specific translation elongation factor (Escherichia coli K-12 substr. MG1655)
3.6.1.15,3.6.5.6,3.6.5.5,3.6.5.4,3.6.5.3,3.6.5.2,3.6.5.1,3.6.1.-,3.6.1.5 [EC: 3.6.1.15, 3.6.1.5, 3.6.5.1, 3.6.5.2, 3.6.5.3, 3.6.5.4, 3.6.5.5, 3.6.5.6]

Predicted SEED Role

"Selenocysteine-specific translation elongation factor" in subsystem Selenocysteine metabolism

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (14/14 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (6/8 steps found)
• UTP and CTP dephosphorylation I (5/7 steps found)
• UTP and CTP dephosphorylation II (1/3 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Pyrimidine metabolism
• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.15

Use Curated BLAST to search for 3.6.1.15 or 3.6.1.5 or 3.6.5.1 or 3.6.5.2 or 3.6.5.3 or 3.6.5.4 or 3.6.5.5 or 3.6.5.6

Protein Sequence (615 amino acids)

>EX28DRAFT_3844 selenocysteine-specific elongation factor SelB (Enterobacter asburiae PDN3)
MIIATAGHVDHGKTTLLQAITGINADRLPEEKKRGMTIDLGYAYWPQPDGRVLGFIDVPG
HEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLTGNPQLTVALTKADRVDDA
RVSEVRETVQATLQEYGFADAPLFVTVATEGRGIDELRHHLQQLSSRDHASHHRFRLAID
RAFTVKGAGLVVTGTALSGEVNVGDTLWLTGVNKPMRVRGLHAQNQPAEHAHAGQRIALN
IAGDAEKEQLNRGDWLLADAPPEPSERVIVSLQSNVPLTQWQPLHIHHAASHITGRVSLL
ENDLAELVFDSPLWLADNDRLVLRDISARETLAGARVVALNPPRRGKRKPEYLQWLAALA
QASDDNAALRVHLERGAVSLRDFAWARQLSEEGLRLLAQQPGFIQAGNSLLNAPVAARWQ
RKILSTLATYHEQHQDEPGPGRERLRRMALPMEDDALVLLLIENMRESGAIKSHHGWLHL
PDHKAGFTAEQEAVWQKVASLFGDEPWWVRDLARETNTDEQVMRQVLRHAAQQGLIVAIV
KDRYYRNDRIVAFANLIRELDQERGSTCAADFRDRLNVGRKLAIQILEYFNRIGFTRRRG
NDHLLRDSLLFPETP