Protein Info for EX28DRAFT_3823 in Enterobacter asburiae PDN3

Annotation: glycyl-tRNA synthetase beta chain (EC 6.1.1.14)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 689 TIGR00211: glycine--tRNA ligase, beta subunit" amino acids 1 to 689 (689 residues), 1081.6 bits, see alignment E=0 PF02092: tRNA_synt_2f" amino acids 6 to 551 (546 residues), 717.2 bits, see alignment E=1.7e-219 PF05746: DALR_1" amino acids 581 to 678 (98 residues), 38.6 bits, see alignment E=1.1e-13

Best Hits

Swiss-Prot: 97% identical to SYGB_ENT38: Glycine--tRNA ligase beta subunit (glyS) from Enterobacter sp. (strain 638)

KEGG orthology group: K01879, glycyl-tRNA synthetase beta chain [EC: 6.1.1.14] (inferred from 98% identity to enc:ECL_00208)

MetaCyc: 94% identical to glycine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Glycine--tRNA ligase. [EC: 6.1.1.14]

Predicted SEED Role

"Glycyl-tRNA synthetase beta chain (EC 6.1.1.14)" (EC 6.1.1.14)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.14

Use Curated BLAST to search for 6.1.1.14

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (689 amino acids)

>EX28DRAFT_3823 glycyl-tRNA synthetase beta chain (EC 6.1.1.14) (Enterobacter asburiae PDN3)
MSEKTFLVEIGTEELPPKALRSLAESFAANVTAELDNAGLAHGKIEWFAAPRRLALKVAN
LAASQPDREVEKRGPAIAQAFDAEGKPSKAAEGWARGCGITVDQAERLTTDKGEWLLYRA
HVKGESAEALLPDMIATSLAKLPIPKLMRWGASDVHFVRPVHTVTLLLGDTVIPATILGV
ASDRVIRGHRFMGEPEFTIDSADQYPQILLERGKVIADYEQRKAKIKADAEEAARQIGGN
ADLSESLLEEVTSLVEWPVVLTAKFEEKFLAVPAEALVYTMKGDQKYFPVYANDGKLLPN
FIFVANIESKDPIQIISGNEKVVRPRLADAEFFFNTDRKKRLEDNLPRLQTVLFQQQLGT
LRDKTDRIAELSGWIAREIGADVNHATRAGLLSKCDLMTNMVFEFTDTQGVMGMHYARHD
GEAEDVAVALNEQYQPRFAGDDLPSNPVACAVAIADKMDTLAGIFGIGQHPKGDKDPFAL
RRAALGVLRIIVEKNLNLDLQTLTEEAVRLYGDKLTNAKVVDDVIDFMLGRFRAWYQDEG
YTVDTIQAVLARRPTRPADFDARMKAVSHFRTLEAASALAAANKRVSNILAKSDETLNER
VNAATLKEPEEIALAMQVVVLRDKLEPYFTEGRYQEALVELAELRDVIDAFFEKVMVNVE
DKELRINRLSMLEKLRELFLRVADISLLQ