Protein Info for EX28DRAFT_3806 in Enterobacter asburiae PDN3

Annotation: Predicted membrane-associated, metal-dependent hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 564 transmembrane" amino acids 12 to 30 (19 residues), see Phobius details amino acids 45 to 66 (22 residues), see Phobius details amino acids 76 to 103 (28 residues), see Phobius details amino acids 123 to 139 (17 residues), see Phobius details amino acids 160 to 177 (18 residues), see Phobius details PF08019: EptA_B_N" amino acids 59 to 171 (113 residues), 51.8 bits, see alignment E=8.5e-18 PF00884: Sulfatase" amino acids 250 to 539 (290 residues), 205.5 bits, see alignment E=1.3e-64

Best Hits

Swiss-Prot: 76% identical to EPTB_ECOLI: Kdo(2)-lipid A phosphoethanolamine 7''-transferase (eptB) from Escherichia coli (strain K12)

KEGG orthology group: K12975, phosphoethanolamine transferase (inferred from 94% identity to enc:ECL_00228)

MetaCyc: 76% identical to phosphoethanolamine transferase EptB (Escherichia coli K-12 substr. MG1655)
RXN-16286 [EC: 2.7.8.42]; 2.7.8.42 [EC: 2.7.8.42]; 2.7.8.42 [EC: 2.7.8.42]

Predicted SEED Role

"Phosphoethanolamine transferase specific for the outer Kdo residue of lipopolysaccharide" in subsystem Lipid A modifications

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.8.42

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (564 amino acids)

>EX28DRAFT_3806 Predicted membrane-associated, metal-dependent hydrolase (Enterobacter asburiae PDN3)
MKYIRSLTQQRLCLMLAVYIGLFLNGAVLFRRVEGYFENLTVSNSIFAAIEVFGSVLATF
FLLRLLSLFGRRTWQILASLVVIISAAASYYMTFMNVVIGYGIVASVMTTDIDLSKEVVG
KGFIVWTVLTCLIPLFFIWNNTCRYTLLRQLRTRGQRIRNIVVVLLAGLLVWAPIRLMET
QQKRVEKATGVDMPSYGGVVANSYLPSNWLSALGLYAWAQADESSDVKSLINPLKKFTYQ
PPADGIDDTYVVFVIGETTRWDHMGILGYDRDTTPKLAQEKNLVAYRGYSCDTATKLSLR
CMFVREGGASDNPQRTLKEQNVFAVLKQLGFSSDLFAMQSEMWFYTNTLADNIAYREQIG
AEPRNRGKNVDDMLLLEEMSQSLKNHPQGKHLVILHTKGSHYSYYQRYTRDFAKWQPECV
GINKDCSKQELINAYDNSVLYVDTFLSRVIDQLRDKKAIVIYAADHGESINEKEHLHGTP
RKLAPPEQFRVPMIMWMSDKYLENPEKAKMFAQLKKEADMKVPRRHVELYDTIMGCLGYT
SPNGGINENNNWCKLPDNSVKAAQ