Protein Info for EX28DRAFT_3781 in Enterobacter asburiae PDN3

Annotation: Uncharacterized lipoprotein NlpE involved in copper resistance

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 232 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details PF04170: NlpE" amino acids 44 to 127 (84 residues), 92.2 bits, see alignment E=3.1e-30 PF17185: NlpE_C" amino acids 142 to 230 (89 residues), 110.2 bits, see alignment E=5.4e-36

Best Hits

Swiss-Prot: 68% identical to NLPE_ECOLI: Lipoprotein NlpE (nlpE) from Escherichia coli (strain K12)

KEGG orthology group: K06079, copper homeostasis protein (lipoprotein) (inferred from 96% identity to enc:ECL_00997)

Predicted SEED Role

"Copper homeostasis protein CutF precursor / Lipoprotein NlpE involeved in surface adhesion" in subsystem Copper homeostasis: copper tolerance

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (232 amino acids)

>EX28DRAFT_3781 Uncharacterized lipoprotein NlpE involved in copper resistance (Enterobacter asburiae PDN3)
MKKVLWSALAASTLFALFGCNNRSETQVLQPTQTEELKPMQQSWRGVLPCADCEGIETSL
FLQKDGTWVMNQRYQGAKEPSSFATYGTWARTAEKLVLTDTTGEKTFFRAKGEGMEMLDR
EGNPIESQFNYTLAPVKAALPATPMAMRGMYFYMADAAIFTDCATGRKVSVANNAQLERD
YAVARGNDSKPVLLTVDGHFTLEPNPDSGEMVKTLVADKDAKFVAGKDCNSK