Protein Info for EX28DRAFT_3739 in Enterobacter asburiae PDN3

Annotation: TonB-dependent siderophore receptor

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 749 signal peptide" amino acids 1 to 35 (35 residues), see Phobius details PF07715: Plug" amino acids 78 to 182 (105 residues), 82.4 bits, see alignment E=3.3e-27 TIGR01783: TonB-dependent siderophore receptor" amino acids 80 to 749 (670 residues), 646.6 bits, see alignment E=2.6e-198 PF00593: TonB_dep_Rec_b-barrel" amino acids 256 to 718 (463 residues), 219.2 bits, see alignment E=2e-68

Best Hits

Swiss-Prot: 88% identical to FHUA_ECOLI: Ferrichrome outer membrane transporter/phage receptor (fhuA) from Escherichia coli (strain K12)

KEGG orthology group: K02014, iron complex outermembrane recepter protein (inferred from 97% identity to enc:ECL_00954)

MetaCyc: 88% identical to ferrichrome outer membrane transporter/phage receptor (Escherichia coli K-12 substr. MG1655)
RXN0-1701

Predicted SEED Role

"Ferric hydroxamate outer membrane receptor FhuA" in subsystem Iron acquisition in Vibrio or Siderophore Aerobactin

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (749 amino acids)

>EX28DRAFT_3739 TonB-dependent siderophore receptor (Enterobacter asburiae PDN3)
MALSNTAQPMNTSLLKLAVVVATAVAGMSAYAHAAEAPKKEETITVTAAPAAQESAWGPA
ATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPRSVKEALSYTPGVAVGTRGASN
TYDYLIIRGFAADGQTQNNYLDGMKMQGNFYNDAVIDPYMLERAEIMRGPVSVLYGKSSP
GGLLNMVSKRPTTEPLKEIQFKVGTDSLFQTGFDFSDAIDDDGVYSYRLTGVARSNNAQQ
ERAEEQRYAIAPSFSWRPDDKTTFTFLSYFQNEPETGYYGWLPKEGTVDPLPNGDRLPTN
FNEGAKNNTYSRNQKMVGYSFDHEFNDTFTVRQNLRFAENKTSQNSVYGYGVCTDPANSG
NKQCAALAPSDKGHYLARKYVVDNEKLQNFTVDTQLQSKFSTGEVDHVLLTGVDFMRMRN
DINSWFGYDDSVPLLDLYNPVNSDFDFSSKDPATSGPYQILNRQKQTGLYVQDQAQWDKV
LVTLGGRYDWADQESYNRVTNTTSKRDDTQFTWRGGVNYLFDNGVTPYFSYSESFEPASQ
TDAQGKLFSPSKGKQYEAGVKYVPNDRPIVVTGALYQLTKTNNLMADPAGSFFSVQGGEI
RARGVEVEAKAALSASVNLVGSYTYTDAEYTTDTTYKGNTPAQVPKHMASLWGDYTLFDG
ALSGLTLGTGVRYTGSSYGDPANSFKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFD
REYVASCFNTYGCFWGAERQVVATATFRF