Protein Info for EX28DRAFT_3738 in Enterobacter asburiae PDN3

Annotation: penicillin-binding protein 1B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 841 transmembrane" amino acids 64 to 85 (22 residues), see Phobius details PF14812: PBP1_TM" amino acids 1 to 78 (78 residues), 98.9 bits, see alignment E=4e-32 TIGR02071: penicillin-binding protein 1B" amino acids 67 to 792 (726 residues), 1242.6 bits, see alignment E=0 PF17757: UvrB_inter" amino acids 110 to 190 (81 residues), 25.4 bits, see alignment E=3.1e-09 PF14814: UB2H" amino acids 112 to 196 (85 residues), 95 bits, see alignment E=5.7e-31 PF00912: Transgly" amino acids 207 to 378 (172 residues), 188 bits, see alignment E=2.6e-59 PF00905: Transpeptidase" amino acids 472 to 711 (240 residues), 98.7 bits, see alignment E=9.3e-32

Best Hits

Swiss-Prot: 90% identical to PBPB_ECOLI: Penicillin-binding protein 1B (mrcB) from Escherichia coli (strain K12)

KEGG orthology group: K05365, penicillin-binding protein 1B [EC: 2.4.1.129 3.4.-.-] (inferred from 99% identity to enc:ECL_00953)

MetaCyc: 90% identical to peptidoglycan glycosyltransferase / peptidoglycan DD-transpeptidase MrcB (Escherichia coli K-12 substr. MG1655)
Serine-type D-Ala-D-Ala carboxypeptidase. [EC: 3.4.16.4]; 3.4.16.4 [EC: 3.4.16.4]; Peptidoglycan glycosyltransferase. [EC: 3.4.16.4, 2.4.1.129]

Predicted SEED Role

"Multimodular transpeptidase-transglycosylase (EC 2.4.1.129) (EC 3.4.-.-)" in subsystem Peptidoglycan Biosynthesis or Type IV pilus (EC 2.4.1.129, EC 3.4.-.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.1.129, 3.4.-.-, 3.4.16.4

Use Curated BLAST to search for 2.4.1.129 or 3.4.-.- or 3.4.16.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (841 amino acids)

>EX28DRAFT_3738 penicillin-binding protein 1B (Enterobacter asburiae PDN3)
MAGNDREPIGRKGKPTRPAKEKVSRRRLRDEDYDDDYEDDYEDEEPMPRNGKGKGRKPRG
KRGWFWLLLKLFIVFVVLMAIYGVYLDQKIRSRIDGKVWQLPAAVYGRMVNLEPDMSITK
NEMVKLLEATQYRQVSKMTRPGEFTVQSNSIEMIRRPFDFPDSKEGQVRARLTFDGDRLD
TIENMDNNRQFGFFRLDPRLITMLSSANGEQRLFVARNGFPDLLVDTLLATEDRHFYEHD
GISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNLFLSSERSYWRKANEAYMAVLMDARY
SKDRILELYMNEVYLGQSGDNEIRGFPLASLYYFGRPVEELSLDQQALLVGMVKGASIYN
PWRNPKLALERRNLVLRLLQQQQVIDQELYDMLSARPLGVQPRGGVISPQPAFMQLVRQE
LQSKLGDKVKDLSGVKIFTTFDSVAQDAAEKAAVEGIPALKKQRKLSDLETAMVVVDRNT
GEVRAMVGGAEPQFAGYNRAMQARRSIGSLAKPATYLTALSQPNQYRLNTWIADAPISLR
QPNGQVWSPQNDDKQFSGQVMLVDALTRSMNVPTVNLGMSLGLPAIVDTWQKLGVSKDQL
HPVPAMILGALNLTPIEVAQAFQTIASGGNRAPLSALRSVIAEDGSVLYQSFPQAERAVP
AQAAYMTLWTMQQVVQRGTGRQLGAKYPGLHLAGKTGTTNNNVDTWFAGIDGREVVITWV
GRDNNQPTKLYGASGAMSIYQRYLANQSPVPLNLVAPEDIVDMGVDSSGNFVCGGGMRTL
PVWTANPDSLCQQSQPEQPTGNPFDQSSQPQQPQQQQPQQQNEKKDSDGVAGWIKDMFGG
N