Protein Info for EX28DRAFT_3737 in Enterobacter asburiae PDN3

Annotation: ATP-dependent helicase HrpB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 809 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details TIGR01970: ATP-dependent helicase HrpB" amino acids 4 to 808 (805 residues), 1194.4 bits, see alignment E=0 PF00270: DEAD" amino acids 16 to 162 (147 residues), 49.2 bits, see alignment E=8e-17 PF00271: Helicase_C" amino acids 206 to 327 (122 residues), 40.7 bits, see alignment E=3.8e-14 PF08482: HrpB_C" amino acids 664 to 796 (133 residues), 180.4 bits, see alignment E=3.2e-57

Best Hits

Swiss-Prot: 82% identical to HRPB_ECOLI: ATP-dependent RNA helicase HrpB (hrpB) from Escherichia coli (strain K12)

KEGG orthology group: K03579, ATP-dependent helicase HrpB [EC: 3.6.4.13] (inferred from 92% identity to enc:ECL_00952)

Predicted SEED Role

"ATP-dependent helicase HrpB"

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.13

Use Curated BLAST to search for 3.6.4.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (809 amino acids)

>EX28DRAFT_3737 ATP-dependent helicase HrpB (Enterobacter asburiae PDN3)
MSSLPVAVILPELLAALHHAPQVLLSAPTGAGKSTWLPLQILKDGNISGKIILLEPRRLA
ARNVAQRLAELLNEKAGETVGYRMRAETCVGPTTRLEVVTEGILTRMLQNDPELNGVGLV
ILDEFHERSLQADLALALLLDVQQGLRDDLRLLIMSATLDNERLRQALPDAPVISSEGRA
FPVERRYQPLPSHQRFDEAVAIATAELLRQEAGSLLLFLPGVGEIQRVQEQLSTRVGGDV
LLCPLYGALSLQEQRKAILPAPAGQRKVVLATNIAETSLTIEGIRLVVDSAQERVASFDP
RTGLTKLLTQRISQASMVQRAGRAGRLEPGICLHLTSAEQAERAAQQSTPEILQSDLSGL
VMDLLQWGYPNLEQLTWLNPPPVVNLTAARTLLTQLGALEGERLTARGQKMAALGNDPRL
AAMLVAAQGDDEIATAAKLAAILEEPPRGGSSDLGQAFSRSQGNWLQRAQQLCKRLNSRG
GAPDSDKIAFLLAQAFPDRIARRRGLDGRYQLANGMGAMLDSDDALTRHEWLIAPLLLQG
SHSPDARILQAIAVEIDALTRACPQLLQQSDTVEWDDAQGTLKAFRRSQVGKLTLGTKPL
AKPSEDELHQAMLNGIREKGLSALNWTPEAEQYRIRLHCAAKWLPDYSWPAVDDETLLAT
LERWLLPQMSGVHSLRALKALDVKTALQNLLDWSLRQRLDSELPGHYTVPTGSRIAIRYH
EDNPPALAVRMQEMFGEATTPSIAEGRVPLVLELLSPAHRPLQITRDLGAFWAGSYREVQ
KEMKGRYPKHVWPDDPANTAPTRRTKKYS